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Ciliary beat frequency and structure of recipient and donor epithelia following lung transplantation

Ciliary beat frequency and structure of recipient and donor epithelia following lung transplantation
Ciliary beat frequency and structure of recipient and donor epithelia following lung transplantation

To investigate possible changes following lung transplantation, the structure and in vitro ciliary beat frequency (CBF) of airway epithelium from the cytology brushings of 9 heart-lung (HLT) and 5 single-lung (SLT) transplant recipients were examined. The CBF of brushings taken proximal and distal to the anastomosis was measured 2-10 months following transplant. There was no difference between the measured mean CBF at the two sites or between the two groups; HLT CBF: distal 11.0 +/- 0.5 Hz (standard error of mean), proximal 10.5 +/- 0.4 Hz, SLT CBF: distal 11.7 +/- 0.9 Hz, proximal 12.0 +/- 0.6 Hz. Mean CBF of bronchial brushings (except distal brushings from SLT patients) was significantly lower than that from controls: 13.6 +/- 0.3 Hz (n = 7) (p less than 0.05). Transmission electron microscopy of epithelial brushings from 4 patients (3 HLT, 1 SLT) revealed epithelial abnormalities both proximal and distal to the anastomosis, particularly ciliary depletion, mitochondrial abnormalities and death of cells. No significant ciliary ultrastructural abnormalities were seen in any tissue. We conclude that epithelial abnormalities were observed both proximal and distal to the anastomosis following lung transplantation. These may contribute to impairment of mucociliary clearance.

Bronchi, Cilia, Epithelium, Heart-Lung Transplantation, Humans, Lung Transplantation, Microscopy, Electron, Mucociliary Clearance, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
0903-1936
796-801
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Shankar, S.
bae66df4-fdb7-429a-9d77-c87417bf5389
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Feldman, C.
f466c1a8-5663-4837-972d-1eb566eff1e5
Yacoub, M.
e556fcba-3454-4330-99ae-54750a23691f
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Shankar, S.
bae66df4-fdb7-429a-9d77-c87417bf5389
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Feldman, C.
f466c1a8-5663-4837-972d-1eb566eff1e5
Yacoub, M.
e556fcba-3454-4330-99ae-54750a23691f
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651

Read, R.C., Shankar, S., Rutman, A., Feldman, C., Yacoub, M., Cole, P.J. and Wilson, R. (1991) Ciliary beat frequency and structure of recipient and donor epithelia following lung transplantation. European Respiratory Journal, 4 (7), 796-801.

Record type: Article

Abstract

To investigate possible changes following lung transplantation, the structure and in vitro ciliary beat frequency (CBF) of airway epithelium from the cytology brushings of 9 heart-lung (HLT) and 5 single-lung (SLT) transplant recipients were examined. The CBF of brushings taken proximal and distal to the anastomosis was measured 2-10 months following transplant. There was no difference between the measured mean CBF at the two sites or between the two groups; HLT CBF: distal 11.0 +/- 0.5 Hz (standard error of mean), proximal 10.5 +/- 0.4 Hz, SLT CBF: distal 11.7 +/- 0.9 Hz, proximal 12.0 +/- 0.6 Hz. Mean CBF of bronchial brushings (except distal brushings from SLT patients) was significantly lower than that from controls: 13.6 +/- 0.3 Hz (n = 7) (p less than 0.05). Transmission electron microscopy of epithelial brushings from 4 patients (3 HLT, 1 SLT) revealed epithelial abnormalities both proximal and distal to the anastomosis, particularly ciliary depletion, mitochondrial abnormalities and death of cells. No significant ciliary ultrastructural abnormalities were seen in any tissue. We conclude that epithelial abnormalities were observed both proximal and distal to the anastomosis following lung transplantation. These may contribute to impairment of mucociliary clearance.

Full text not available from this repository.

More information

Published date: July 1991
Keywords: Bronchi, Cilia, Epithelium, Heart-Lung Transplantation, Humans, Lung Transplantation, Microscopy, Electron, Mucociliary Clearance, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 417002
URI: https://eprints.soton.ac.uk/id/eprint/417002
ISSN: 0903-1936
PURE UUID: 13b9bafc-44c6-4b81-beb2-01c8c4df9597
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 16 Jan 2018 17:30
Last modified: 14 Mar 2019 01:36

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