In vitro and in vivo effects of ribavirin on human respiratory epithelium
In vitro and in vivo effects of ribavirin on human respiratory epithelium
The effects of ribavirin, a broad spectrum antiviral agent, on the structure and function of normal human nasal epithelium have been studied in vitro, as has also the in vivo effect of treatment with nebulised ribavirin on nasal mucociliary clearance of saccharin in four patients. Ciliary beat frequency was measured by a photometric technique, and changes in epithelial and ciliary ultrastructure were assessed by transmission electron microscopy. Ribavirin solution at the recommended concentration of 20 mg/ml had no adverse effects on ciliary activity in vitro; at concentrations of 50 mg/ml and above it slowed ciliary beating significantly and at 60 mg/ml caused ciliostasis associated with epithelial disruption. Nasal inhalation of ribavirin at 60 mg/ml for up to 20 minutes, however, did not slow nasal mucociliary clearance, nor did it adversely affect the ciliary beating or structure of nasal ciliated epithelium examined in vitro immediately after inhalation.
Aged, Cilia, Epithelium, Female, Humans, Male, Microscopy, Electron, Middle Aged, Mucociliary Clearance, Nasal Mucosa, Ribavirin, Ribonucleosides, Journal Article, Research Support, Non-U.S. Gov't
100-104
Han, L.Y.
492397ae-3d9d-49a7-8a59-69766aa90b12
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Slater, S.
a15800cf-54cd-470b-8def-32873789e58d
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Snell, N.J.
7bed5a8b-462f-4863-9002-b53bf88b48e5
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
February 1990
Han, L.Y.
492397ae-3d9d-49a7-8a59-69766aa90b12
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Slater, S.
a15800cf-54cd-470b-8def-32873789e58d
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Snell, N.J.
7bed5a8b-462f-4863-9002-b53bf88b48e5
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Han, L.Y., Wilson, R., Slater, S., Rutman, A., Read, R.C., Snell, N.J. and Cole, P.J.
(1990)
In vitro and in vivo effects of ribavirin on human respiratory epithelium.
Thorax, 45 (2), .
(doi:10.1136/thx.45.2.100).
Abstract
The effects of ribavirin, a broad spectrum antiviral agent, on the structure and function of normal human nasal epithelium have been studied in vitro, as has also the in vivo effect of treatment with nebulised ribavirin on nasal mucociliary clearance of saccharin in four patients. Ciliary beat frequency was measured by a photometric technique, and changes in epithelial and ciliary ultrastructure were assessed by transmission electron microscopy. Ribavirin solution at the recommended concentration of 20 mg/ml had no adverse effects on ciliary activity in vitro; at concentrations of 50 mg/ml and above it slowed ciliary beating significantly and at 60 mg/ml caused ciliostasis associated with epithelial disruption. Nasal inhalation of ribavirin at 60 mg/ml for up to 20 minutes, however, did not slow nasal mucociliary clearance, nor did it adversely affect the ciliary beating or structure of nasal ciliated epithelium examined in vitro immediately after inhalation.
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Published date: February 1990
Keywords:
Aged, Cilia, Epithelium, Female, Humans, Male, Microscopy, Electron, Middle Aged, Mucociliary Clearance, Nasal Mucosa, Ribavirin, Ribonucleosides, Journal Article, Research Support, Non-U.S. Gov't
Identifiers
Local EPrints ID: 417041
URI: http://eprints.soton.ac.uk/id/eprint/417041
ISSN: 0040-6376
PURE UUID: 3a5929b6-e534-470b-bc50-2b76c08cda1b
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Date deposited: 17 Jan 2018 17:30
Last modified: 16 Mar 2024 04:10
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Contributors
Author:
L.Y. Han
Author:
R. Wilson
Author:
S. Slater
Author:
A. Rutman
Author:
N.J. Snell
Author:
P.J. Cole
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