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Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path

Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path
Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path
We report on the use of a laser-direct write (LDW) technique that allows the fabrication of lateral flow devices with enhanced sensitivity and limit of detection. This technique comprises the deposition of a photopolymer at specific regions of a nitrocellulose membrane and its subsequent photopolymerisation to create impenetrable walls inside the volume of the membrane. These polymerised structures are intentionally designed to create fluidic channels which are constricted over a specific length that spans the test zone within which the sample interacts with pre-deposited reagents. The slower flow rate and smaller test zone area result in the increased sensitivity and lowered limit of detection for these devices, and these are quantified via the improved performance of a C-Reactive Protein (CRP) assay. Finally, it is demonstrated that the sensitivity of the CRP assay is improved by 62x and its limit of detection by 30x
1473-0197
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
He, Peijun
2e303166-6aa5-4a09-b22e-440d96a54a9f
Eason, Robert
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
He, Peijun
2e303166-6aa5-4a09-b22e-440d96a54a9f
Eason, Robert
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

Katis, Ioannis, He, Peijun, Eason, Robert and Sones, Collin (2018) Improved sensitivity and limit-of-detection of lateral flow devices using spatial constrictions of the flow-path Lab on a Chip

Record type: Article

Abstract

We report on the use of a laser-direct write (LDW) technique that allows the fabrication of lateral flow devices with enhanced sensitivity and limit of detection. This technique comprises the deposition of a photopolymer at specific regions of a nitrocellulose membrane and its subsequent photopolymerisation to create impenetrable walls inside the volume of the membrane. These polymerised structures are intentionally designed to create fluidic channels which are constricted over a specific length that spans the test zone within which the sample interacts with pre-deposited reagents. The slower flow rate and smaller test zone area result in the increased sensitivity and lowered limit of detection for these devices, and these are quantified via the improved performance of a C-Reactive Protein (CRP) assay. Finally, it is demonstrated that the sensitivity of the CRP assay is improved by 62x and its limit of detection by 30x

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More information

In preparation date: 2018

Identifiers

Local EPrints ID: 417094
URI: https://eprints.soton.ac.uk/id/eprint/417094
ISSN: 1473-0197
PURE UUID: 8c07e802-392c-4ac7-8af0-642c94650dd3
ORCID for Ioannis Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for Robert Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 19 Jan 2018 17:30
Last modified: 19 Jan 2018 17:30

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Contributors

Author: Ioannis Katis ORCID iD
Author: Peijun He
Author: Robert Eason ORCID iD
Author: Collin Sones

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