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Mechano-sensitization of mammalian neuronal networks through expression of the bacterial mechanosensitive MscL channel

Mechano-sensitization of mammalian neuronal networks through expression of the bacterial mechanosensitive MscL channel
Mechano-sensitization of mammalian neuronal networks through expression of the bacterial mechanosensitive MscL channel
Development of remote stimulation techniques for neuronal tissues represents a challenging goal. Among the potential methods, mechanical stimuli are the most promising vector to convey information non-invasively into intact brain tissue. In this context, selective mechano-sensitization of neuronal circuits would pave the way to develop a new cell-type specific stimulation approach. We report here for the first time the development and characterization of mechano-sensitized neuronal networks through the heterologous expression of an engineered bacterial large conductance mechanosensitive ion channel (MscL). The neuronal functional expression of the MscL channel was validated through patch-clamp recordings upon application of calibrated suction pressures. Moreover, we verified the effective development of in-vitro neuronal networks expressing the engineered MscL channel in terms of cell survival, number of synaptic puncta, and spontaneous network activity. The pure mechanosensitivity of the engineered MscL channel, with its wide genetic modification library, may represent a versatile tool to further develop a mechano-genetic approach.
Nanopore engineering , Neuronal mechano-sensitization, Mechanobiology, MscL, Exclusively mechanosensitive ion channel
0021-9533
Soloperto, Alessandro
50836aa2-b615-48e1-8dc0-0010e047706f
Boccaccio, Anna
be93c2b7-c992-4f72-876c-fce8a2e8196e
Contestabile, Andrea
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Moroni, Monica
8ca48a7c-7f58-4a2e-8e9b-bb5a0cc196d7
Hallinan, Grace
5549f978-4afd-4b1c-a4e1-6fe6530d3573
Palazzolo, Gemma
2bd702b4-ff21-4a3f-973b-4922c6f9f6b4
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Difato, Francesco
2eb3a7b9-8fa6-46c4-a33d-e5c74598d6cb
Soloperto, Alessandro
50836aa2-b615-48e1-8dc0-0010e047706f
Boccaccio, Anna
be93c2b7-c992-4f72-876c-fce8a2e8196e
Contestabile, Andrea
e18a59be-f6c3-4818-935c-f619869d3ce2
Moroni, Monica
8ca48a7c-7f58-4a2e-8e9b-bb5a0cc196d7
Hallinan, Grace
5549f978-4afd-4b1c-a4e1-6fe6530d3573
Palazzolo, Gemma
2bd702b4-ff21-4a3f-973b-4922c6f9f6b4
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Difato, Francesco
2eb3a7b9-8fa6-46c4-a33d-e5c74598d6cb

Soloperto, Alessandro, Boccaccio, Anna, Contestabile, Andrea, Moroni, Monica, Hallinan, Grace, Palazzolo, Gemma, Chad, John, Deinhardt, Katrin, Carugo, Dario and Difato, Francesco (2018) Mechano-sensitization of mammalian neuronal networks through expression of the bacterial mechanosensitive MscL channel. Journal of Cell Science, 131 (5), [jcs210393]. (doi:10.1242/jcs.210393).

Record type: Article

Abstract

Development of remote stimulation techniques for neuronal tissues represents a challenging goal. Among the potential methods, mechanical stimuli are the most promising vector to convey information non-invasively into intact brain tissue. In this context, selective mechano-sensitization of neuronal circuits would pave the way to develop a new cell-type specific stimulation approach. We report here for the first time the development and characterization of mechano-sensitized neuronal networks through the heterologous expression of an engineered bacterial large conductance mechanosensitive ion channel (MscL). The neuronal functional expression of the MscL channel was validated through patch-clamp recordings upon application of calibrated suction pressures. Moreover, we verified the effective development of in-vitro neuronal networks expressing the engineered MscL channel in terms of cell survival, number of synaptic puncta, and spontaneous network activity. The pure mechanosensitivity of the engineered MscL channel, with its wide genetic modification library, may represent a versatile tool to further develop a mechano-genetic approach.

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Accepted/In Press date: 13 January 2018
e-pub ahead of print date: 19 January 2018
Published date: 8 March 2018
Keywords: Nanopore engineering , Neuronal mechano-sensitization, Mechanobiology, MscL, Exclusively mechanosensitive ion channel

Identifiers

Local EPrints ID: 417105
URI: http://eprints.soton.ac.uk/id/eprint/417105
ISSN: 0021-9533
PURE UUID: 84e301ac-fcb1-4e38-8cd8-6d0083d474bc
ORCID for John Chad: ORCID iD orcid.org/0000-0001-6442-4281
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298

Catalogue record

Date deposited: 19 Jan 2018 17:30
Last modified: 16 Mar 2024 06:07

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Contributors

Author: Anna Boccaccio
Author: Andrea Contestabile
Author: Monica Moroni
Author: Grace Hallinan
Author: Gemma Palazzolo
Author: John Chad ORCID iD
Author: Dario Carugo
Author: Francesco Difato

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