The University of Southampton
University of Southampton Institutional Repository

Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells

Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells
Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells
The transcriptional repressor Blimp-1 originally cloned as a silencer of type I interferon (IFN)-β gene expression controls cell fate decisions in multiple tissue contexts. Conditional inactivation in the mammary gland was recently shown to disrupt epithelial cell architecture. Here we report that Blimp-1 regulates expression of viral defense, IFN signaling and MHC class I pathways, and directly targets the transcriptional activator Stat1. Blimp-1 functional loss in 3D cultures of mammary epithelial cells (MECs) results in accumulation of dsRNA and expression of type III IFN-λ. Cultures treated with IFN lambda similarly display defective lumen formation. These results demonstrate that type III IFN-λ profoundly influences the behavior of MECs and identify Blimp-1 as a critical regulator of IFN signaling cascades.
2045-2322
1-11
Elias, Salah
a9b11116-8efb-44b3-8241-2f0f2af847c3
Robertson, Elizabeth J.
80d0f6ff-a50b-4fe4-a968-281d74b1e5de
Bikoff, Elizabeth K.
d58c427d-46a2-4567-9077-554ab6894da7
Mould, Arne W.
a913032e-73dc-419c-bbd7-b2aaba22f495
Elias, Salah
a9b11116-8efb-44b3-8241-2f0f2af847c3
Robertson, Elizabeth J.
80d0f6ff-a50b-4fe4-a968-281d74b1e5de
Bikoff, Elizabeth K.
d58c427d-46a2-4567-9077-554ab6894da7
Mould, Arne W.
a913032e-73dc-419c-bbd7-b2aaba22f495

Elias, Salah, Robertson, Elizabeth J., Bikoff, Elizabeth K. and Mould, Arne W. (2018) Blimp-1/PRDM1 is a critical regulator of Type III Interferon responses in mammary epithelial cells. Scientific Reports, 8 (1), 1-11. (doi:10.1038/s41598-017-18652-9).

Record type: Article

Abstract

The transcriptional repressor Blimp-1 originally cloned as a silencer of type I interferon (IFN)-β gene expression controls cell fate decisions in multiple tissue contexts. Conditional inactivation in the mammary gland was recently shown to disrupt epithelial cell architecture. Here we report that Blimp-1 regulates expression of viral defense, IFN signaling and MHC class I pathways, and directly targets the transcriptional activator Stat1. Blimp-1 functional loss in 3D cultures of mammary epithelial cells (MECs) results in accumulation of dsRNA and expression of type III IFN-λ. Cultures treated with IFN lambda similarly display defective lumen formation. These results demonstrate that type III IFN-λ profoundly influences the behavior of MECs and identify Blimp-1 as a critical regulator of IFN signaling cascades.

Text s41598-017-18652-9 - Version of Record
Available under License Creative Commons Attribution.
Download (4MB)

More information

Accepted/In Press date: 15 December 2017
e-pub ahead of print date: 10 January 2018

Identifiers

Local EPrints ID: 417175
URI: https://eprints.soton.ac.uk/id/eprint/417175
ISSN: 2045-2322
PURE UUID: c0a2ae92-b337-4224-990f-c21137545643

Catalogue record

Date deposited: 23 Jan 2018 17:30
Last modified: 24 Jan 2018 17:30

Export record

Altmetrics

Contributors

Author: Salah Elias
Author: Elizabeth J. Robertson
Author: Elizabeth K. Bikoff
Author: Arne W. Mould

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×