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Detecting and identifying DNA via the THz backbone frequency using a metamaterial-based label-free biosensor

Detecting and identifying DNA via the THz backbone frequency using a metamaterial-based label-free biosensor
Detecting and identifying DNA via the THz backbone frequency using a metamaterial-based label-free biosensor
In genetic diagnostics, laboratory-based equipment generally uses analytical techniques requiring complicated and expensive fluorescent labelling of target DNA molecules. Intense research effort into, and commercial development of, Point-of-Care diagnostics and Personalized Healthcare are driving the development of simple, fast and cost-effective detection methods. One potential label-free DNA detection method uses Terahertz (THz) spectroscopy of the natural responses of DNA in metamaterial structures, which are engineered to have properties that are impossible to obtain in natural materials. This paper presents a study of the development of metamaterials based on asymmetric X-shaped resonator inclusions as a functional sensor for DNA. Gold X-shaped resonator structures with dimensions of 90/85 μm were demonstrated to produce trapped mode resonant frequency in the correct range for DNA detection. Realistic substrate materials in the form of 375 μm thick quartz were investigated, demonstrating that the non-transparent nature of the material resulted in the production of standing waves, affecting the system response, as well as requiring a reduction in scale of the resonator of 85%. As a result, the effect of introducing etched windows in the substrate material were investigated, demonstrating that increased window size significantly reduces the effect of the substrate on the system response. The device design showed a good selectivity when RNA samples were introduced to the model, demonstrating the potential for this design of device in the development of sensors capable of performing cheap and simple genetic analysis of DNA, giving label-free detection at high sensitivity.
SPIE
Mirzaei, Sahar
a400fcd0-346c-4abd-b667-6c7012fc84f1
Green, Nicolas
d9b47269-c426-41fd-a41d-5f4579faa581
Rotaru, Mihai
c53c5038-2fed-4ace-8fad-9f95d4c95b7e
Pu, Suan-Hui
8b46b970-56fd-4a4e-8688-28668f648f43
Sadwick, Laurence P.
Yan, Tianxin
Mirzaei, Sahar
a400fcd0-346c-4abd-b667-6c7012fc84f1
Green, Nicolas
d9b47269-c426-41fd-a41d-5f4579faa581
Rotaru, Mihai
c53c5038-2fed-4ace-8fad-9f95d4c95b7e
Pu, Suan-Hui
8b46b970-56fd-4a4e-8688-28668f648f43
Sadwick, Laurence P.
Yan, Tianxin

Mirzaei, Sahar, Green, Nicolas, Rotaru, Mihai and Pu, Suan-Hui (2017) Detecting and identifying DNA via the THz backbone frequency using a metamaterial-based label-free biosensor. Sadwick, Laurence P. and Yan, Tianxin (eds.) In Terahertz, RF, Millimeter, and Submillimeter-Wave Technology and Applications X. vol. 10103, SPIE. 9 pp . (doi:10.1117/12.2263694).

Record type: Conference or Workshop Item (Paper)

Abstract

In genetic diagnostics, laboratory-based equipment generally uses analytical techniques requiring complicated and expensive fluorescent labelling of target DNA molecules. Intense research effort into, and commercial development of, Point-of-Care diagnostics and Personalized Healthcare are driving the development of simple, fast and cost-effective detection methods. One potential label-free DNA detection method uses Terahertz (THz) spectroscopy of the natural responses of DNA in metamaterial structures, which are engineered to have properties that are impossible to obtain in natural materials. This paper presents a study of the development of metamaterials based on asymmetric X-shaped resonator inclusions as a functional sensor for DNA. Gold X-shaped resonator structures with dimensions of 90/85 μm were demonstrated to produce trapped mode resonant frequency in the correct range for DNA detection. Realistic substrate materials in the form of 375 μm thick quartz were investigated, demonstrating that the non-transparent nature of the material resulted in the production of standing waves, affecting the system response, as well as requiring a reduction in scale of the resonator of 85%. As a result, the effect of introducing etched windows in the substrate material were investigated, demonstrating that increased window size significantly reduces the effect of the substrate on the system response. The device design showed a good selectivity when RNA samples were introduced to the model, demonstrating the potential for this design of device in the development of sensors capable of performing cheap and simple genetic analysis of DNA, giving label-free detection at high sensitivity.

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More information

e-pub ahead of print date: 24 February 2017
Published date: 11 May 2017
Venue - Dates: SPIE Photonics West 2017, San Francisco, United States, 2017-01-27 - 2017-02-01

Identifiers

Local EPrints ID: 417257
URI: http://eprints.soton.ac.uk/id/eprint/417257
PURE UUID: 140ca26c-5691-478a-b089-baf1dac23ce4
ORCID for Sahar Mirzaei: ORCID iD orcid.org/0000-0001-7474-5492
ORCID for Nicolas Green: ORCID iD orcid.org/0000-0001-9230-4455
ORCID for Suan-Hui Pu: ORCID iD orcid.org/0000-0002-3335-8880

Catalogue record

Date deposited: 26 Jan 2018 17:30
Last modified: 17 Dec 2019 01:47

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Contributors

Author: Sahar Mirzaei ORCID iD
Author: Nicolas Green ORCID iD
Author: Mihai Rotaru
Author: Suan-Hui Pu ORCID iD
Editor: Laurence P. Sadwick
Editor: Tianxin Yan

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