Achieving biocompatible SABRE: An invitro cytotoxicity study
Achieving biocompatible SABRE: An invitro cytotoxicity study
Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.
Biocompatibility, Biphasic catalysis, Cytotoxicity, Hyperpolarization, SABRE
Manoharan, Anand
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Rayner, Peter J.
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Iali, Wissam
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Burns, Michael J.
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Perry, V. Hugh
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Duckett, Simon B.
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Manoharan, Anand
ae3918f7-54d5-4321-9e41-18179b0052bf
Rayner, Peter J.
91603c52-4e8e-418c-b7d5-25cead3a8e49
Iali, Wissam
74b43f75-005f-4d8a-b5b5-1b9aff3d2c13
Burns, Michael J.
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Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Duckett, Simon B.
f0cf4d7b-14f8-42cb-9117-0ffca9593652
Manoharan, Anand, Rayner, Peter J., Iali, Wissam, Burns, Michael J., Perry, V. Hugh and Duckett, Simon B.
(2018)
Achieving biocompatible SABRE: An invitro cytotoxicity study.
ChemMedChem.
(doi:10.1002/cmdc.201700725).
Abstract
Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.
Text
Manoharan_et_al-2018-ChemMedChem
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Accepted/In Press date: 12 December 2017
e-pub ahead of print date: 18 January 2018
Keywords:
Biocompatibility, Biphasic catalysis, Cytotoxicity, Hyperpolarization, SABRE
Identifiers
Local EPrints ID: 417418
URI: http://eprints.soton.ac.uk/id/eprint/417418
ISSN: 1860-7179
PURE UUID: e6987fb1-9801-44eb-80e3-cceca220b6a9
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Date deposited: 31 Jan 2018 17:30
Last modified: 15 Mar 2024 18:11
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Contributors
Author:
Anand Manoharan
Author:
Peter J. Rayner
Author:
Wissam Iali
Author:
Michael J. Burns
Author:
Simon B. Duckett
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