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Achieving biocompatible SABRE: An invitro cytotoxicity study

Achieving biocompatible SABRE: An invitro cytotoxicity study
Achieving biocompatible SABRE: An invitro cytotoxicity study

Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.

Biocompatibility, Biphasic catalysis, Cytotoxicity, Hyperpolarization, SABRE
1860-7179
Manoharan, Anand
ae3918f7-54d5-4321-9e41-18179b0052bf
Rayner, Peter J.
91603c52-4e8e-418c-b7d5-25cead3a8e49
Iali, Wissam
74b43f75-005f-4d8a-b5b5-1b9aff3d2c13
Burns, Michael J.
d1cbc9ad-42df-492c-b3f3-235419edf4a3
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Duckett, Simon B.
f0cf4d7b-14f8-42cb-9117-0ffca9593652
Manoharan, Anand
ae3918f7-54d5-4321-9e41-18179b0052bf
Rayner, Peter J.
91603c52-4e8e-418c-b7d5-25cead3a8e49
Iali, Wissam
74b43f75-005f-4d8a-b5b5-1b9aff3d2c13
Burns, Michael J.
d1cbc9ad-42df-492c-b3f3-235419edf4a3
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Duckett, Simon B.
f0cf4d7b-14f8-42cb-9117-0ffca9593652

Manoharan, Anand, Rayner, Peter J., Iali, Wissam, Burns, Michael J., Perry, V. Hugh and Duckett, Simon B. (2018) Achieving biocompatible SABRE: An invitro cytotoxicity study. ChemMedChem. (doi:10.1002/cmdc.201700725).

Record type: Article

Abstract

Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.

Text Manoharan_et_al-2018-ChemMedChem - Version of Record
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More information

Accepted/In Press date: 12 December 2017
e-pub ahead of print date: 18 January 2018
Keywords: Biocompatibility, Biphasic catalysis, Cytotoxicity, Hyperpolarization, SABRE

Identifiers

Local EPrints ID: 417418
URI: https://eprints.soton.ac.uk/id/eprint/417418
ISSN: 1860-7179
PURE UUID: e6987fb1-9801-44eb-80e3-cceca220b6a9

Catalogue record

Date deposited: 31 Jan 2018 17:30
Last modified: 31 Jan 2018 17:30

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