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MicroRNA 8059 as a marker for the presence and extent of coronary artery calcification

MicroRNA 8059 as a marker for the presence and extent of coronary artery calcification
MicroRNA 8059 as a marker for the presence and extent of coronary artery calcification
Objective: microRNAs (miRNAs) may serve as potential biomarkers in a variety of pathologies. The aim of this study was to determine whether miRNAs could serve as blood-based markers of isolated coronary artery calcification (CAC) defined as CAC in the absence of an underlying metabolic abnormality. Methods: 24 age-matched and sex-matched patients who had been referred for elective CT coronary calcium score and angiography as part of investigation for cardiac chest pain were recruited. Peripheral venesection was performed and an Agatston calcium score was derived from the CT coronary angiogram using default software. RNA was extracted using the LeukoLOCK Total RNA Isolation System for Toray’s microarray analysis and quantitative reverse transcription PCR (qRT-PCR). Results: the patients were well matched for age, sex and conventional risk factors for coronary artery disease. Microarray analysis identified lower expression of miRNA-138-2-3p, miRNA-1181, miRNA-6816-3p and miRNA-8059 in patients with coronary artery calcium score (CACS)=0 vs CACS>100. qRT-PCR confirmed significant downregulation of miRNA-8059 in patients with CACS>100 (CACS=0 vs CACS>100; P=0.03). Conclusion: miRNA-8059 may serve as a peripheral blood-based biomarker for the presence of CAC, as well as provide a platform for studying the pathophysiological basis of isolated CAC.
microRNA, coronary artery calcification
Howlett, Philippa
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Cleal, Jane
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Wu, Huihai
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Shah, Nikunj
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Horton, Alex
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Curzen, Nicholas
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Mahmoudi, Michael
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Howlett, Philippa
d0ed92bf-ed27-41c5-a6d5-48c9f9c14eff
Cleal, Jane
18cfd2c1-bd86-4a13-b38f-c321af56da66
Wu, Huihai
0dd13617-4769-4144-b7cd-061b19bc0917
Shah, Nikunj
f6769b48-013f-4e31-929d-7a6882b4af7b
Horton, Alex
cf7f099f-bbb6-4f5c-9b99-49f23b2ff14e
Curzen, Nicholas
70f3ea49-51b1-418f-8e56-8210aef1abf4
Mahmoudi, Michael
f6a55246-399e-4f81-944e-a4b169786e8a

Howlett, Philippa, Cleal, Jane, Wu, Huihai, Shah, Nikunj, Horton, Alex, Curzen, Nicholas and Mahmoudi, Michael (2018) MicroRNA 8059 as a marker for the presence and extent of coronary artery calcification. Open Heart, 5 (1), [e000678]. (doi:10.1136/openhrt-2017-000678).

Record type: Article

Abstract

Objective: microRNAs (miRNAs) may serve as potential biomarkers in a variety of pathologies. The aim of this study was to determine whether miRNAs could serve as blood-based markers of isolated coronary artery calcification (CAC) defined as CAC in the absence of an underlying metabolic abnormality. Methods: 24 age-matched and sex-matched patients who had been referred for elective CT coronary calcium score and angiography as part of investigation for cardiac chest pain were recruited. Peripheral venesection was performed and an Agatston calcium score was derived from the CT coronary angiogram using default software. RNA was extracted using the LeukoLOCK Total RNA Isolation System for Toray’s microarray analysis and quantitative reverse transcription PCR (qRT-PCR). Results: the patients were well matched for age, sex and conventional risk factors for coronary artery disease. Microarray analysis identified lower expression of miRNA-138-2-3p, miRNA-1181, miRNA-6816-3p and miRNA-8059 in patients with coronary artery calcium score (CACS)=0 vs CACS>100. qRT-PCR confirmed significant downregulation of miRNA-8059 in patients with CACS>100 (CACS=0 vs CACS>100; P=0.03). Conclusion: miRNA-8059 may serve as a peripheral blood-based biomarker for the presence of CAC, as well as provide a platform for studying the pathophysiological basis of isolated CAC.

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Accepted/In Press date: 11 December 2017
e-pub ahead of print date: 31 January 2018
Published date: 31 January 2018
Keywords: microRNA, coronary artery calcification

Identifiers

Local EPrints ID: 417886
URI: http://eprints.soton.ac.uk/id/eprint/417886
PURE UUID: 07cb9f10-16fc-4d14-ba7e-02a181eecc18
ORCID for Jane Cleal: ORCID iD orcid.org/0000-0001-7978-4327
ORCID for Nicholas Curzen: ORCID iD orcid.org/0000-0001-9651-7829
ORCID for Michael Mahmoudi: ORCID iD orcid.org/0000-0003-1293-8461

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Date deposited: 16 Feb 2018 17:30
Last modified: 26 Nov 2021 03:05

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Contributors

Author: Philippa Howlett
Author: Jane Cleal ORCID iD
Author: Huihai Wu
Author: Nikunj Shah
Author: Alex Horton
Author: Nicholas Curzen ORCID iD

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