Marchan, Rosemarie, Büttner, Bettina, Lambert, Jörg, Edlund, Karolina, Glaeser, Iris, Blaszkewicz, Meinolf, Leonhardt, Gregor, Marienhoff, Lisa, Kaszta, Darius, Anft, Moritz, Watzl, Carsten, Madjar, Katrin, Grinberg, Marianna, Rempel, Eugen, Hergenröder, Roland, Selinski, Silvia, Rahnenführer, Jörg, Lesjak, Michaela S, Stewart, Joanna D, Cadenas, Cristina and Hengstler, Jan G. (2017) Glycerol-3-phosphate acyltransferase 1 promotes tumor cell migration and poor survival in ovarian carcinoma. Cancer Research, 77 (17), 4589-4601. (doi:10.1158/0008-5472.CAN-16-2065).
Abstract
Glycerophosphodiesterase EDI3 (GPCPD1; GDE5; GDPD6) has been suggested to promote cell migration, adhesion, and spreading, but its mechanisms of action remain uncertain. In this study, we targeted the glycerol-3-phosphate acyltransferase GPAM along with choline kinase-α (CHKA), the enzymes that catabolize the products of EDI3 to determine which downstream pathway is relevant for migration. Our results clearly showed that GPAM influenced cell migration via the signaling lipid lysophosphatidic acid (LPA), linking it with GPAM to cell migration. Analysis of GPAM expression in different cancer types revealed a significant association between high GPAM expression and reduced overall survival in ovarian cancer. Silencing GPAM in ovarian cancer cells decreased cell migration and reduced the growth of tumor xenografts. In contrast to these observations, manipulating CHKA did not influence cell migration in the same set of cell lines. Overall, our findings show how GPAM influences intracellular LPA levels to promote cell migration and tumor growth.Cancer Res; 77(17); 4589-601. ©2017 AACR.
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