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Inhibition of intra-Golgi transport in vitro by mitotic kinase

Inhibition of intra-Golgi transport in vitro by mitotic kinase
Inhibition of intra-Golgi transport in vitro by mitotic kinase

It has previously been shown that exocytic and endocytic membrane traffic are inhibited in mitotic mammalian cells. Here we have used a cell-free intra-Golgi transport assay supplemented with heterologous cytosols to mimic this effect in vitro. Cytosols with high histone kinase activity, made either from mitotic cells or by cyclin A treatment of interphase cells, inhibited intra-Golgi transport by up to 75%. Inhibition of transport was reversed by the kinase inhibitor staurosporine or by reduction in ATP levels leading to inactivation of histone kinase. The data indicate that cell cycle control of intra-Golgi transport is due to a reversible modification of cytosol, and this assay system may be used to study the molecular mechanism of mitotic transport inhibition in mammalian cells.

Alkaloids, Animals, Biological Transport, CHO Cells, Cell-Free System, Cricetinae, Cytosol, Golgi Apparatus, HeLa Cells, Humans, Mitosis, Protamine Kinase, Staurosporine, Journal Article
0021-9258
4050-4
Stuart, R A
e5cfc779-258c-43ab-a22e-8ada3b22f761
Mackay, D
588a653e-9785-4a00-be71-4e547850ee4a
Adamczewski, J
5af1636c-877f-4904-8d70-54020de0a6c9
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a
Stuart, R A
e5cfc779-258c-43ab-a22e-8ada3b22f761
Mackay, D
588a653e-9785-4a00-be71-4e547850ee4a
Adamczewski, J
5af1636c-877f-4904-8d70-54020de0a6c9
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a

Stuart, R A, Mackay, D, Adamczewski, J and Warren, Graham (1993) Inhibition of intra-Golgi transport in vitro by mitotic kinase. The Journal of Biological Chemistry, 268 (6), 4050-4.

Record type: Article

Abstract

It has previously been shown that exocytic and endocytic membrane traffic are inhibited in mitotic mammalian cells. Here we have used a cell-free intra-Golgi transport assay supplemented with heterologous cytosols to mimic this effect in vitro. Cytosols with high histone kinase activity, made either from mitotic cells or by cyclin A treatment of interphase cells, inhibited intra-Golgi transport by up to 75%. Inhibition of transport was reversed by the kinase inhibitor staurosporine or by reduction in ATP levels leading to inactivation of histone kinase. The data indicate that cell cycle control of intra-Golgi transport is due to a reversible modification of cytosol, and this assay system may be used to study the molecular mechanism of mitotic transport inhibition in mammalian cells.

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More information

Published date: 25 February 1993
Keywords: Alkaloids, Animals, Biological Transport, CHO Cells, Cell-Free System, Cricetinae, Cytosol, Golgi Apparatus, HeLa Cells, Humans, Mitosis, Protamine Kinase, Staurosporine, Journal Article

Identifiers

Local EPrints ID: 418319
URI: https://eprints.soton.ac.uk/id/eprint/418319
ISSN: 0021-9258
PURE UUID: 55d4d3fe-4278-46cf-95dd-a916339bf6fe
ORCID for D Mackay: ORCID iD orcid.org/0000-0003-3088-4401

Catalogue record

Date deposited: 28 Feb 2018 17:30
Last modified: 14 Mar 2019 01:48

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