Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2
Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2
An in vitro assay was used to study the role of p34cdc2 in cyclin A-mediated vesicular transport inhibition. It was shown that the S-phase kinase p33cdk2 reduced the effect of cyclin A on transport assays performed with sHeLa cytosol, even though histone kinase was strongly activated. Also, transport with FT210 cytosol (which is temperature-sensitive for p34cdc2) was inhibited by cyclin A only at the permissive temperature. However, the phosphatase inhibitor microcystin inhibited transport without any requirement for p34cdc2 activity. These results show that transport is inhibited by cyclin A via p34cdc2, and also by another kinase, possibly downstream of p34cdc2.
Alkaloids, Animals, Biological Transport, CDC2 Protein Kinase, CHO Cells, Cell Line, Cricetinae, Cyclins, Cytosol, Golgi Apparatus, HeLa Cells, Humans, Microcystins, Peptides, Cyclic, Phosphoprotein Phosphatases, Protamine Kinase, Protein Kinase C, Staurosporine, Journal Article
549-54
Mackay, D
588a653e-9785-4a00-be71-4e547850ee4a
Kieckbusch, Regina
ae07b6ee-7ab5-44b2-a0e6-2d54a3517f88
Adamczewski, Jorg
5af1636c-877f-4904-8d70-54020de0a6c9
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a
28 December 1993
Mackay, D
588a653e-9785-4a00-be71-4e547850ee4a
Kieckbusch, Regina
ae07b6ee-7ab5-44b2-a0e6-2d54a3517f88
Adamczewski, Jorg
5af1636c-877f-4904-8d70-54020de0a6c9
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a
Mackay, D, Kieckbusch, Regina, Adamczewski, Jorg and Warren, Graham
(1993)
Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2.
FEBS Letters, 336 (3), .
(doi:10.1016/0014-5793(93)80874-T).
Abstract
An in vitro assay was used to study the role of p34cdc2 in cyclin A-mediated vesicular transport inhibition. It was shown that the S-phase kinase p33cdk2 reduced the effect of cyclin A on transport assays performed with sHeLa cytosol, even though histone kinase was strongly activated. Also, transport with FT210 cytosol (which is temperature-sensitive for p34cdc2) was inhibited by cyclin A only at the permissive temperature. However, the phosphatase inhibitor microcystin inhibited transport without any requirement for p34cdc2 activity. These results show that transport is inhibited by cyclin A via p34cdc2, and also by another kinase, possibly downstream of p34cdc2.
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Published date: 28 December 1993
Keywords:
Alkaloids, Animals, Biological Transport, CDC2 Protein Kinase, CHO Cells, Cell Line, Cricetinae, Cyclins, Cytosol, Golgi Apparatus, HeLa Cells, Humans, Microcystins, Peptides, Cyclic, Phosphoprotein Phosphatases, Protamine Kinase, Protein Kinase C, Staurosporine, Journal Article
Identifiers
Local EPrints ID: 418320
URI: http://eprints.soton.ac.uk/id/eprint/418320
ISSN: 0014-5793
PURE UUID: 4f804347-ba48-45aa-97c5-b4699f741641
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Date deposited: 28 Feb 2018 17:30
Last modified: 16 Mar 2024 03:05
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Author:
Regina Kieckbusch
Author:
Jorg Adamczewski
Author:
Graham Warren
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