Investigating the stability of DNA-coated gold nanoparticles
Investigating the stability of DNA-coated gold nanoparticles
In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
February 2018
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Kyriazi, Maria-Eleni and Kanaras, Antonios
(2018)
Investigating the stability of DNA-coated gold nanoparticles.
In Colloidal Nanoparticles for Biomedical Applications XIII.
vol. 10507,
SPIE..
(doi:10.1117/12.2285833).
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Abstract
In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.
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Published date: February 2018
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Local EPrints ID: 418507
URI: http://eprints.soton.ac.uk/id/eprint/418507
PURE UUID: c99e688d-d079-45fb-9ee6-bf73c15e7d2b
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Date deposited: 09 Mar 2018 17:30
Last modified: 16 Mar 2024 03:56
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Author:
Maria-Eleni Kyriazi
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