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Investigating the stability of DNA-coated gold nanoparticles

Investigating the stability of DNA-coated gold nanoparticles
Investigating the stability of DNA-coated gold nanoparticles
In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.
SPIE: the International Society for Optics and Photonics
Kyriazi, Maria-Eleni
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Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7

Kyriazi, Maria-Eleni and Kanaras, Antonios (2018) Investigating the stability of DNA-coated gold nanoparticles. In Colloidal Nanoparticles for Biomedical Applications XIII. vol. 10507, SPIE: the International Society for Optics and Photonics.. (doi:10.1117/12.2285833).

Record type: Conference or Workshop Item (Paper)

Abstract

In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.

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Published date: February 2018

Identifiers

Local EPrints ID: 418507
URI: https://eprints.soton.ac.uk/id/eprint/418507
PURE UUID: c99e688d-d079-45fb-9ee6-bf73c15e7d2b
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 09 Mar 2018 17:30
Last modified: 14 Mar 2019 01:39

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