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Spectroscopic and hydrodynamic characterisation of DNA-linked gold nanoparticle dimers in solution using two-photon photoluminescence

Spectroscopic and hydrodynamic characterisation of DNA-linked gold nanoparticle dimers in solution using two-photon photoluminescence
Spectroscopic and hydrodynamic characterisation of DNA-linked gold nanoparticle dimers in solution using two-photon photoluminescence
Two-photon photoluminescence (TPPL) emission spectra of DNA–gold nanoparticle (AuNP) monoconjugates and the corresponding DNA-linked AuNP dimers are obtained by photon time-of-flight spectroscopy. This technique is combined with two-photon photoluminescence fluctuation correlation spectroscopy (TPPL–FCS) to simultaneously monitor the optical and hydrodynamic behaviour of these nano-assemblies in solution, with single-particle sensitivity and microsecond temporal resolution. In this study, the AuNPs have an average core diameter of 12 nm, which renders their dark-field plasmonic light scattering too weak for single-particle imaging. Moreover, as a result of the lack of plasmonic coupling in the dimers, the optical extinction, scattering and photoluminescence spectra of the DNA–AuNP complexes are not sufficiently different to distinguish between monomers and dimers. The use of TPPL–FCS successfully addresses these bottlenecks and enables the distinction between AuNP monomers and AuNP dimers in solution by measurement of their hydrodynamic rotational and translational diffusion.
1439-4235
Midelet, Johanna
9ead62c9-7a1c-4f84-81f3-918e3683b909
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Debarre, Anne
ac68a0e6-6121-4c0b-ab8e-c850245b4528
Werts, Martinus H.V.
750f9843-296d-40e6-9ef0-75cae4a042cd
Midelet, Johanna
9ead62c9-7a1c-4f84-81f3-918e3683b909
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Debarre, Anne
ac68a0e6-6121-4c0b-ab8e-c850245b4528
Werts, Martinus H.V.
750f9843-296d-40e6-9ef0-75cae4a042cd

Midelet, Johanna, El-Sagheer, Afaf, Brown, Tom, Kanaras, Antonios, Debarre, Anne and Werts, Martinus H.V. (2018) Spectroscopic and hydrodynamic characterisation of DNA-linked gold nanoparticle dimers in solution using two-photon photoluminescence. ChemPhysChem. (doi:10.1002/cphc.201701228).

Record type: Article

Abstract

Two-photon photoluminescence (TPPL) emission spectra of DNA–gold nanoparticle (AuNP) monoconjugates and the corresponding DNA-linked AuNP dimers are obtained by photon time-of-flight spectroscopy. This technique is combined with two-photon photoluminescence fluctuation correlation spectroscopy (TPPL–FCS) to simultaneously monitor the optical and hydrodynamic behaviour of these nano-assemblies in solution, with single-particle sensitivity and microsecond temporal resolution. In this study, the AuNPs have an average core diameter of 12 nm, which renders their dark-field plasmonic light scattering too weak for single-particle imaging. Moreover, as a result of the lack of plasmonic coupling in the dimers, the optical extinction, scattering and photoluminescence spectra of the DNA–AuNP complexes are not sufficiently different to distinguish between monomers and dimers. The use of TPPL–FCS successfully addresses these bottlenecks and enables the distinction between AuNP monomers and AuNP dimers in solution by measurement of their hydrodynamic rotational and translational diffusion.

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Accepted/In Press date: 28 January 2018
e-pub ahead of print date: 21 February 2018

Identifiers

Local EPrints ID: 418609
URI: https://eprints.soton.ac.uk/id/eprint/418609
ISSN: 1439-4235
PURE UUID: 6afc34c9-8f41-4b4e-b4bc-32b664ee5198
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 12 Mar 2018 17:30
Last modified: 14 Mar 2019 05:13

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