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An advanced magnetic resonance imaging perspective on the etiology of deep tissue injury: MRI of deep tissue injury etiology

An advanced magnetic resonance imaging perspective on the etiology of deep tissue injury: MRI of deep tissue injury etiology
An advanced magnetic resonance imaging perspective on the etiology of deep tissue injury: MRI of deep tissue injury etiology
Early diagnosis of deep tissue injury remains problematic due to the complicated and multi-factorial nature of damage induction, and the many processes involved in damage development and recovery. In this paper we present a comprehensive assessment of deep tissue injury development and remodeling in a rat model by multi-parametric magnetic resonance imaging (MRI) and histopathology. The tibialis anterior muscle of rats was subjected to mechanical deformation for 2 h. Multi-parametric in vivo MRI, consisting of T2, T2∗, mean diffusivity (MD), and angiography measurements, was applied before, during, and directly after indentation, as well as at several time points during a 14 days follow-up. MRI readouts were linked to histological analyses of the damaged tissue. The results showed dynamic change in various MRI parameters, reflecting the histopathological status of the tissue during damage induction and repair. Increased T2 corresponded with edema, muscle cell damage, and inflammation. T2∗ was related to tissue perfusion, hemorrhage, and inflammation. MD increase and decrease reported on the tissue's microstructural integrity and reflected muscle degeneration, edema, as well as fibrosis. Angiography provided information on blockage of blood flow during deformation. Our results indicate that the effects of a single damage causing event of only 2 h deformation were present up to 14 days. The initial tissue response to deformation, as observed by MRI, starts at the edge of the indentation. The quantitative MRI readouts provided distinct and complementary information on the extent, temporal evolution, and microstructural basis of deep tissue injury related muscle damage.
8750-7587
1580-1596
Nelissen, Jules Laurent
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Traa, Willeke A.
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de Boer, Hans H.
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de Graaf, Larry
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Mazzoli, Valentina
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Savci-Heijink, C. Dilara
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Nicolay, Klaas
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Froeling, Martijn
a26359b7-faca-4e83-a242-be7df6b04265
Bader, Daniel
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Nederveen, Aart J.
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Oomens, Cees W. J.
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Strijkers, Gustav J.
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Nelissen, Jules Laurent
d7ade1c4-43ea-4331-b623-67d229300720
Traa, Willeke A.
7133843c-25e6-456b-b472-a70e15ba9f28
de Boer, Hans H.
31b275fa-2277-4bd0-9851-22e299f91bb9
de Graaf, Larry
f13eed1d-e8bb-41f7-9b82-68de5407e6b9
Mazzoli, Valentina
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Savci-Heijink, C. Dilara
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Nicolay, Klaas
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Froeling, Martijn
a26359b7-faca-4e83-a242-be7df6b04265
Bader, Daniel
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Nederveen, Aart J.
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Oomens, Cees W. J.
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Strijkers, Gustav J.
c8506691-27fd-48f5-9ba9-174ba3feed28

Nelissen, Jules Laurent, Traa, Willeke A., de Boer, Hans H., de Graaf, Larry, Mazzoli, Valentina, Savci-Heijink, C. Dilara, Nicolay, Klaas, Froeling, Martijn, Bader, Daniel, Nederveen, Aart J., Oomens, Cees W. J. and Strijkers, Gustav J. (2018) An advanced magnetic resonance imaging perspective on the etiology of deep tissue injury: MRI of deep tissue injury etiology. Journal of Applied Physiology, 124 (6), 1580-1596. (doi:10.1152/japplphysiol.00891.2017).

Record type: Article

Abstract

Early diagnosis of deep tissue injury remains problematic due to the complicated and multi-factorial nature of damage induction, and the many processes involved in damage development and recovery. In this paper we present a comprehensive assessment of deep tissue injury development and remodeling in a rat model by multi-parametric magnetic resonance imaging (MRI) and histopathology. The tibialis anterior muscle of rats was subjected to mechanical deformation for 2 h. Multi-parametric in vivo MRI, consisting of T2, T2∗, mean diffusivity (MD), and angiography measurements, was applied before, during, and directly after indentation, as well as at several time points during a 14 days follow-up. MRI readouts were linked to histological analyses of the damaged tissue. The results showed dynamic change in various MRI parameters, reflecting the histopathological status of the tissue during damage induction and repair. Increased T2 corresponded with edema, muscle cell damage, and inflammation. T2∗ was related to tissue perfusion, hemorrhage, and inflammation. MD increase and decrease reported on the tissue's microstructural integrity and reflected muscle degeneration, edema, as well as fibrosis. Angiography provided information on blockage of blood flow during deformation. Our results indicate that the effects of a single damage causing event of only 2 h deformation were present up to 14 days. The initial tissue response to deformation, as observed by MRI, starts at the edge of the indentation. The quantitative MRI readouts provided distinct and complementary information on the extent, temporal evolution, and microstructural basis of deep tissue injury related muscle damage.

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An advanced magnetic resonance imaging perspective on the etiology of deep tissue injury - Accepted Manuscript
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Accepted/In Press date: 20 February 2018
e-pub ahead of print date: 28 June 2018
Published date: June 2018

Identifiers

Local EPrints ID: 418680
URI: http://eprints.soton.ac.uk/id/eprint/418680
ISSN: 8750-7587
PURE UUID: dede7227-c584-4777-8f83-bda85bd63f37
ORCID for Daniel Bader: ORCID iD orcid.org/0000-0002-1208-3507

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Date deposited: 16 Mar 2018 17:30
Last modified: 26 Nov 2021 07:06

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Contributors

Author: Jules Laurent Nelissen
Author: Willeke A. Traa
Author: Hans H. de Boer
Author: Larry de Graaf
Author: Valentina Mazzoli
Author: C. Dilara Savci-Heijink
Author: Klaas Nicolay
Author: Martijn Froeling
Author: Daniel Bader ORCID iD
Author: Aart J. Nederveen
Author: Cees W. J. Oomens
Author: Gustav J. Strijkers

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