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MiR-422a weakened breast cancer stem cells properties by targeting PLP2

MiR-422a weakened breast cancer stem cells properties by targeting PLP2
MiR-422a weakened breast cancer stem cells properties by targeting PLP2
Objective: This study investigated miR-422a and PLP2 expressions in breast cancer cells and breast cancer stem cells (BCSCs). Besides, their influences on polymorphism changes were observed.

Methods: Flow cytometry and fluorescence-activated cell sorting was performed and CD24−/CD44+ cells were sorted from breast cancer cells and recognized as BCSCs. Microarray was applied to search for the differentially expressed miRNAs and mRNAs between MCF7 and BCSCs. The aberrant expression of miR-422a and PLP2 was further confirmed by RT-qPCR and the direct targeted relationship was verified by dual-luciferase reporter assay. After in vitro transfection, the expression of miR-422a and PLP2 were manipulated and biological functions of BMSCs were compared with CCK-8, colony formation and sphere formation assay. The tumorigenesis ability of transfected BMSCs was also investigated in NOD/SCID tumor mice models.

Results:
BMSCs were successfully established from MCF7 cells and miR-422a expression was downregulated while PLP2 level decreased in BMSCs. MiR-422a directly targets the 3’UTR of PLP2 and suppressed its expression. Besides, the up-regulation of miR-422a contributed to weakened ability of proliferation and microsphere formation of BMSCs, while PLP2 overexpression facilitated those biological abilities. Tumorigenesis of BMSCs in mice models was impaired by either overexpression of miR-442a or silencing of PLP2.

Conclusion: Up-regulation of miR-422a attenuated microsphere formation, proliferation and tumor formation of breast cancer stem cells via suppressing the PLP2 expression.
1538-4047
Zhou, Yanmei
ff7ac115-e20e-436a-92ce-b8549e1d1c5c
Chen, Yuandong
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Yao, Shuo
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Deng, Guangrui
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Liu, Dian
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Yuan, Xun
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Liu, Shunfang
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Rao, Jie
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Xiong, Huihua
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Yuan, Xianglin
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Yu, Shiying
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Zhu, Feng
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Wang, Yihua
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Xiong, Hua
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Zhou, Yanmei
ff7ac115-e20e-436a-92ce-b8549e1d1c5c
Chen, Yuandong
7ebecf7d-e4ca-4426-a2e2-ad57b985304c
Yao, Shuo
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Deng, Guangrui
283f3df4-ac9a-4bee-8a5f-b9c6d43d5f3a
Liu, Dian
3241a84f-857f-4f49-99a0-cc2cab5c7b87
Yuan, Xun
fa4b07f9-2593-4d7f-9ee0-43facb1962aa
Liu, Shunfang
5e9467ab-26db-4973-aa5a-fdfabd928022
Rao, Jie
bb3f3914-400b-4965-bfd0-0f438374164b
Xiong, Huihua
21295efb-d20b-46f1-80c0-f95fcf5d68c3
Yuan, Xianglin
a4472b4e-fae6-4bcc-bd28-15e24614a185
Yu, Shiying
cc1d5284-a233-4626-8e85-1f1e366916d7
Zhu, Feng
c770f586-10eb-4861-9226-418cb29dee55
Wang, Yihua
f5044a95-60a7-42d2-87d6-5f1f789e3a7e
Xiong, Hua
52d37b17-6b0c-4239-bbef-656232a3e31b

Zhou, Yanmei, Chen, Yuandong, Yao, Shuo, Deng, Guangrui, Liu, Dian, Yuan, Xun, Liu, Shunfang, Rao, Jie, Xiong, Huihua, Yuan, Xianglin, Yu, Shiying, Zhu, Feng, Wang, Yihua and Xiong, Hua (2018) MiR-422a weakened breast cancer stem cells properties by targeting PLP2. Cancer Biology & Therapy. (doi:10.1080/15384047.2018.1433497).

Record type: Article

Abstract

Objective: This study investigated miR-422a and PLP2 expressions in breast cancer cells and breast cancer stem cells (BCSCs). Besides, their influences on polymorphism changes were observed.

Methods: Flow cytometry and fluorescence-activated cell sorting was performed and CD24−/CD44+ cells were sorted from breast cancer cells and recognized as BCSCs. Microarray was applied to search for the differentially expressed miRNAs and mRNAs between MCF7 and BCSCs. The aberrant expression of miR-422a and PLP2 was further confirmed by RT-qPCR and the direct targeted relationship was verified by dual-luciferase reporter assay. After in vitro transfection, the expression of miR-422a and PLP2 were manipulated and biological functions of BMSCs were compared with CCK-8, colony formation and sphere formation assay. The tumorigenesis ability of transfected BMSCs was also investigated in NOD/SCID tumor mice models.

Results:
BMSCs were successfully established from MCF7 cells and miR-422a expression was downregulated while PLP2 level decreased in BMSCs. MiR-422a directly targets the 3’UTR of PLP2 and suppressed its expression. Besides, the up-regulation of miR-422a contributed to weakened ability of proliferation and microsphere formation of BMSCs, while PLP2 overexpression facilitated those biological abilities. Tumorigenesis of BMSCs in mice models was impaired by either overexpression of miR-442a or silencing of PLP2.

Conclusion: Up-regulation of miR-422a attenuated microsphere formation, proliferation and tumor formation of breast cancer stem cells via suppressing the PLP2 expression.

Text
MiR 422a weakened breast cancer stem Source Cancer Biol Ther SO 2018 Mar 6 0[PMIDT29509055] - Accepted Manuscript
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Accepted/In Press date: 23 January 2018
e-pub ahead of print date: 6 March 2018

Identifiers

Local EPrints ID: 418744
URI: http://eprints.soton.ac.uk/id/eprint/418744
ISSN: 1538-4047
PURE UUID: 447f1933-41fa-4fa4-9a52-e6aedac773fe
ORCID for Yihua Wang: ORCID iD orcid.org/0000-0001-5561-0648

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Date deposited: 21 Mar 2018 17:30
Last modified: 16 Mar 2024 06:23

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Contributors

Author: Yanmei Zhou
Author: Yuandong Chen
Author: Shuo Yao
Author: Guangrui Deng
Author: Dian Liu
Author: Xun Yuan
Author: Shunfang Liu
Author: Jie Rao
Author: Huihua Xiong
Author: Xianglin Yuan
Author: Shiying Yu
Author: Feng Zhu
Author: Yihua Wang ORCID iD
Author: Hua Xiong

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