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Isomer information from ion mobility separation of high-mannose glycan fragments

Isomer information from ion mobility separation of high-mannose glycan fragments
Isomer information from ion mobility separation of high-mannose glycan fragments
Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans. Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers. For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures. Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra. Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.
1044-0305
972–988
Harvey, David J.
261201b9-2ec2-4f49-bd32-eadac805da98
Seabright, Gemma E.
09e75998-09b0-465f-a059-c89b07f3b2c3
Vasiljevic, Snezana
17e075b4-520d-4b9b-a4a7-08ac394ae5e1
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Struwe, Weston B.
16a348b1-3921-4a2d-b5fb-d341fccea65f
Harvey, David J.
261201b9-2ec2-4f49-bd32-eadac805da98
Seabright, Gemma E.
09e75998-09b0-465f-a059-c89b07f3b2c3
Vasiljevic, Snezana
17e075b4-520d-4b9b-a4a7-08ac394ae5e1
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Struwe, Weston B.
16a348b1-3921-4a2d-b5fb-d341fccea65f

Harvey, David J., Seabright, Gemma E., Vasiljevic, Snezana, Crispin, Max and Struwe, Weston B. (2018) Isomer information from ion mobility separation of high-mannose glycan fragments. Journal of the American Society for Mass Spectrometry, 29 (5), 972–988. (doi:10.1007/s13361-018-1890-5).

Record type: Article

Abstract

Extracted arrival time distributions of negative ion CID-derived fragments produced prior to traveling-wave ion mobility separation were evaluated for their ability to provide structural information on N-linked glycans. Fragmentation of high-mannose glycans released from several glycoproteins, including those from viral sources, provided over 50 fragments, many of which gave unique collisional cross-sections and provided additional information used to assign structural isomers. For example, cross-ring fragments arising from cleavage of the reducing terminal GlcNAc residue on Man8GlcNAc2 isomers have unique collision cross-sections enabling isomers to be differentiated in mixtures. Specific fragment collision cross-sections enabled identification of glycans, the antennae of which terminated in the antigenic α-galactose residue, and ions defining the composition of the 6-antenna of several of the glycans were also found to have different cross-sections from isomeric ions produced in the same spectra. Potential mechanisms for the formation of the various ions are discussed and the estimated collisional cross-sections are tabulated.

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More information

Accepted/In Press date: 8 January 2018
e-pub ahead of print date: 5 March 2018
Published date: May 2018

Identifiers

Local EPrints ID: 418792
URI: http://eprints.soton.ac.uk/id/eprint/418792
ISSN: 1044-0305
PURE UUID: 9b9c7f94-47e4-4f9c-858d-65302cb3c57c
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 22 Mar 2018 17:30
Last modified: 26 Nov 2021 03:11

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Contributors

Author: David J. Harvey
Author: Gemma E. Seabright
Author: Snezana Vasiljevic
Author: Max Crispin ORCID iD
Author: Weston B. Struwe

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