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Cells on hierarchically-structured platforms hosting functionalized nanoparticles

Cells on hierarchically-structured platforms hosting functionalized nanoparticles
Cells on hierarchically-structured platforms hosting functionalized nanoparticles
In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogenously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on the hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NPs-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.
Simitzi, Chara
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Harimech, Pascal K.
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Spanou, Syragw
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Lanara, Christina
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Heuer-Jungemann, Amelie
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Manousaki, Aleka
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Fotakis, Costas
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Ranella, Anthi
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Kanaras, Antonios
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Stratakis, Emmanuel
49667b24-2a90-458f-b906-02823d9f4c85
Simitzi, Chara
6877caa8-cd54-4f60-8595-831ba0722a0b
Harimech, Pascal K.
ff4e2019-889f-40ba-85fd-33b90b8bf4be
Spanou, Syragw
5e7b8181-196e-45fb-b8ed-34317da20d8c
Lanara, Christina
60efe34f-b2b8-46c7-a3cd-be02663263f7
Heuer-Jungemann, Amelie
f9d95f30-be78-49dc-880b-babed9bd2a3c
Manousaki, Aleka
07891aa0-136b-4da5-8c11-59d28e554223
Fotakis, Costas
9561a891-8147-4322-b0d9-365f7abdf136
Ranella, Anthi
bdca535c-c5ab-4162-9065-e96d29b1d709
Kanaras, Antonios
667ecfdc-7647-4bd8-be03-a47bf32504c7
Stratakis, Emmanuel
49667b24-2a90-458f-b906-02823d9f4c85

Simitzi, Chara, Harimech, Pascal K., Spanou, Syragw, Lanara, Christina, Heuer-Jungemann, Amelie, Manousaki, Aleka, Fotakis, Costas, Ranella, Anthi, Kanaras, Antonios and Stratakis, Emmanuel (2018) Cells on hierarchically-structured platforms hosting functionalized nanoparticles. Biomaterials Science, (6). (doi:10.1039/c7bm00904f).

Record type: Article

Abstract

In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogenously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on the hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NPs-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.

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Accepted/In Press date: 12 March 2018
e-pub ahead of print date: 13 March 2018
Published date: 2018

Identifiers

Local EPrints ID: 418800
URI: https://eprints.soton.ac.uk/id/eprint/418800
PURE UUID: 65e4e3da-92dd-4a72-a652-6307f519ed31
ORCID for Antonios Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 22 Mar 2018 17:30
Last modified: 19 Jul 2019 06:00

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