Simitzi, Chara, Harimech, Pascal K., Spanou, Syragw, Lanara, Christina, Heuer-Jungemann, Amelie, Manousaki, Aleka, Fotakis, Costas, Ranella, Anthi, Kanaras, Antonios and Stratakis, Emmanuel (2018) Cells on hierarchically-structured platforms hosting functionalized nanoparticles. Biomaterials Science, (6). (doi:10.1039/c7bm00904f).
Abstract
In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogenously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on the hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NPs-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.
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- Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Physical Sciences and Engineering (pre 2018 reorg) > Physics & Astronomy (pre 2018 reorg) > Quantum, Light & Matter Group (pre 2018 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Physics and Astronomy > Physics & Astronomy (pre 2018 reorg) > Quantum, Light & Matter Group (pre 2018 reorg)
School of Physics and Astronomy > Physics & Astronomy (pre 2018 reorg) > Quantum, Light & Matter Group (pre 2018 reorg)
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