Technical challenges of working with extracellular vesicles
Technical challenges of working with extracellular vesicles
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
881-906
Ramirez, Marcel I.
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Amorim, Maria G.
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Gadelha, Catarina
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Milic, Ivana
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Welsh, Joshua A.
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Freitas, Vanessa M.
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Nawaz, Muhammad
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Akbar, Naveed
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Couch, Yvonne
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Makin, Laura
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Cooke, Fiona
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Vettore, Andre L.
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Batista, Patricia X.
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Freezor, Roberta
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Pezuk, Julia A.
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Rosa-Fernandes, Lívia
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Carreira, Ana Claudia O.
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Devitt, Andrew
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Jacobs, Laura
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Silva, Israel T.
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Coakley, Gillian
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Nunes, Diana N.
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Carter, Dave
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Palmisano, Giuseppe
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Dias-Neto, Emmanuel
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21 January 2018
Ramirez, Marcel I.
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Amorim, Maria G.
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Gadelha, Catarina
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Milic, Ivana
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Welsh, Joshua A.
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Freitas, Vanessa M.
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Nawaz, Muhammad
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Akbar, Naveed
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Couch, Yvonne
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Makin, Laura
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Cooke, Fiona
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Vettore, Andre L.
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Batista, Patricia X.
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Freezor, Roberta
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Pezuk, Julia A.
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Rosa-Fernandes, Lívia
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Carreira, Ana Claudia O.
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Devitt, Andrew
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Jacobs, Laura
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Silva, Israel T.
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Coakley, Gillian
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Nunes, Diana N.
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Carter, Dave
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Palmisano, Giuseppe
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Dias-Neto, Emmanuel
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Ramirez, Marcel I., Amorim, Maria G., Gadelha, Catarina, Milic, Ivana, Welsh, Joshua A., Freitas, Vanessa M., Nawaz, Muhammad, Akbar, Naveed, Couch, Yvonne, Makin, Laura, Cooke, Fiona, Vettore, Andre L., Batista, Patricia X., Freezor, Roberta, Pezuk, Julia A., Rosa-Fernandes, Lívia, Carreira, Ana Claudia O., Devitt, Andrew, Jacobs, Laura, Silva, Israel T., Coakley, Gillian, Nunes, Diana N., Carter, Dave, Palmisano, Giuseppe and Dias-Neto, Emmanuel
(2018)
Technical challenges of working with extracellular vesicles.
Nanoscale, 10 (3), .
(doi:10.1039/c7nr08360b).
Abstract
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
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Accepted/In Press date: 8 December 2017
e-pub ahead of print date: 13 December 2017
Published date: 21 January 2018
Identifiers
Local EPrints ID: 418899
URI: http://eprints.soton.ac.uk/id/eprint/418899
ISSN: 2040-3364
PURE UUID: 60b4095f-2ec7-42ef-8bce-84c7e514fc48
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Date deposited: 23 Mar 2018 17:31
Last modified: 05 Jun 2024 19:20
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Contributors
Author:
Marcel I. Ramirez
Author:
Maria G. Amorim
Author:
Catarina Gadelha
Author:
Ivana Milic
Author:
Joshua A. Welsh
Author:
Vanessa M. Freitas
Author:
Muhammad Nawaz
Author:
Naveed Akbar
Author:
Yvonne Couch
Author:
Laura Makin
Author:
Fiona Cooke
Author:
Andre L. Vettore
Author:
Patricia X. Batista
Author:
Roberta Freezor
Author:
Julia A. Pezuk
Author:
Lívia Rosa-Fernandes
Author:
Ana Claudia O. Carreira
Author:
Andrew Devitt
Author:
Laura Jacobs
Author:
Israel T. Silva
Author:
Gillian Coakley
Author:
Diana N. Nunes
Author:
Dave Carter
Author:
Giuseppe Palmisano
Author:
Emmanuel Dias-Neto
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