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Brief communication. A novel method to visualise the three-dimensional organisation of the human cerebral cortical vasculature

Brief communication. A novel method to visualise the three-dimensional organisation of the human cerebral cortical vasculature
Brief communication. A novel method to visualise the three-dimensional organisation of the human cerebral cortical vasculature

Current tissue-clearing protocols for imaging in three dimensions (3D) are typically applied to optimally fixed, small-volume rodent brain tissue - which is not representative of the tissue found in diagnostic neuropathology laboratories. We present a method to visualise the cerebral cortical vasculature in 3D in human post-mortem brain tissue which had been preserved in formalin for many years. Tissue blocks of cerebral cortex from two control cases, two Alzheimer's brains and two cases from Alzheimer's patients immunised against Aβ42were stained with fluorescent Lycopersicon esculentum agglutinin (Tomato lectin), dehydrated and cleared using an adapted three-dimensional imaging of solvent cleared organs (3DISCO) protocol to visualise the vascular endothelium. Tissue was imaged using light sheet and confocal microscopy and reconstructed in 3D using amira software. The method permits visualisation of the arrangement of the parallel penetrating cortical vasculature in the human brain. The presence of four vascular features including anastomosis, U-shaped vessels, spiralling and loops were revealed. In summary, we present a low cost and simple method to visualise the human cerebral vasculature in 3D compatible with prolonged fixation times (years), allowing study of vascular involvement in a range of normative and pathological states.

Journal Article
0021-8782
1-6
Harrison, C H
b239a10b-65f8-4e01-a7a6-b27bbd7c82bf
Buckland, G R
49af30d7-376f-4b32-b3cf-0bcda0251218
Brooks, S E
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Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Chatelet, D S
6371fd7a-e274-4738-9ccb-3dd4dab32928
Liu, A K L
d320f4df-d374-4fb8-9d63-20bddd3416a4
Gentleman, S M
d28d75f7-05cf-4bca-8f3a-db15ce846466
Boche, D
bdcca10e-6302-4dd0-919f-67218f7e0d61
Nicoll, J A R
88c0685f-000e-4eb7-8f72-f36b4985e8ed
Harrison, C H
b239a10b-65f8-4e01-a7a6-b27bbd7c82bf
Buckland, G R
49af30d7-376f-4b32-b3cf-0bcda0251218
Brooks, S E
4b0ecd34-a592-46b3-a21f-f83bdda46c7b
Johnston, D A
b41163c9-b9d2-425c-af99-2a357204014e
Chatelet, D S
6371fd7a-e274-4738-9ccb-3dd4dab32928
Liu, A K L
d320f4df-d374-4fb8-9d63-20bddd3416a4
Gentleman, S M
d28d75f7-05cf-4bca-8f3a-db15ce846466
Boche, D
bdcca10e-6302-4dd0-919f-67218f7e0d61
Nicoll, J A R
88c0685f-000e-4eb7-8f72-f36b4985e8ed

Harrison, C H, Buckland, G R, Brooks, S E, Johnston, D A, Chatelet, D S, Liu, A K L, Gentleman, S M, Boche, D and Nicoll, J A R (2018) Brief communication. A novel method to visualise the three-dimensional organisation of the human cerebral cortical vasculature. Journal of Anatomy, 1-6. (doi:10.1111/joa.12805).

Record type: Article

Abstract

Current tissue-clearing protocols for imaging in three dimensions (3D) are typically applied to optimally fixed, small-volume rodent brain tissue - which is not representative of the tissue found in diagnostic neuropathology laboratories. We present a method to visualise the cerebral cortical vasculature in 3D in human post-mortem brain tissue which had been preserved in formalin for many years. Tissue blocks of cerebral cortex from two control cases, two Alzheimer's brains and two cases from Alzheimer's patients immunised against Aβ42were stained with fluorescent Lycopersicon esculentum agglutinin (Tomato lectin), dehydrated and cleared using an adapted three-dimensional imaging of solvent cleared organs (3DISCO) protocol to visualise the vascular endothelium. Tissue was imaged using light sheet and confocal microscopy and reconstructed in 3D using amira software. The method permits visualisation of the arrangement of the parallel penetrating cortical vasculature in the human brain. The presence of four vascular features including anastomosis, U-shaped vessels, spiralling and loops were revealed. In summary, we present a low cost and simple method to visualise the human cerebral vasculature in 3D compatible with prolonged fixation times (years), allowing study of vascular involvement in a range of normative and pathological states.

Text
JANAT-2018-0011 Main Document Revisions - Accepted Manuscript
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More information

Accepted/In Press date: 15 February 2018
e-pub ahead of print date: 9 March 2018
Additional Information: © 2018 Anatomical Society.
Keywords: Journal Article

Identifiers

Local EPrints ID: 419003
URI: https://eprints.soton.ac.uk/id/eprint/419003
ISSN: 0021-8782
PURE UUID: 3d183a86-4188-4aa8-93da-22ddcfe00215
ORCID for D A Johnston: ORCID iD orcid.org/0000-0001-6703-6014
ORCID for D Boche: ORCID iD orcid.org/0000-0002-5884-130X
ORCID for J A R Nicoll: ORCID iD orcid.org/0000-0002-9444-7246

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Date deposited: 27 Mar 2018 16:30
Last modified: 20 Jul 2019 04:26

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