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Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis

Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis
Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

0095-1137
2601-2604
Rogers, G.B.
9a939cbd-f83a-41ce-8ba4-a71065e0b87e
Carroll, M.P.
b836d262-6b07-4006-9c81-26653a26588b
Serisier, D.J.
b3eb6ed8-7fa5-4310-a2e6-72d2aa0e2bf9
Hockey, P.M.
4b9a830a-bd84-420a-89c9-4821b3cb97af
Jones, G.
45c8de30-811d-4c88-b8b4-9300f94ff6e7
Kehagia, V.
170c3b61-da9d-4069-8cf5-3fdf5ccdf5aa
Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Bruce, K.D.
1decbcba-5842-4d38-ae16-b540b7972dc6
Rogers, G.B.
9a939cbd-f83a-41ce-8ba4-a71065e0b87e
Carroll, M.P.
b836d262-6b07-4006-9c81-26653a26588b
Serisier, D.J.
b3eb6ed8-7fa5-4310-a2e6-72d2aa0e2bf9
Hockey, P.M.
4b9a830a-bd84-420a-89c9-4821b3cb97af
Jones, G.
45c8de30-811d-4c88-b8b4-9300f94ff6e7
Kehagia, V.
170c3b61-da9d-4069-8cf5-3fdf5ccdf5aa
Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Bruce, K.D.
1decbcba-5842-4d38-ae16-b540b7972dc6

Rogers, G.B., Carroll, M.P., Serisier, D.J., Hockey, P.M., Jones, G., Kehagia, V., Connett, G.J. and Bruce, K.D. (2006) Use of 16S rRNA gene profiling by terminal restriction fragment length polymorphism analysis to compare bacterial communities in sputum and mouthwash samples from patients with cystic fibrosis. Journal of Clinical Microbiology, 44 (7), 2601-2604. (doi:10.1128/JCM.02282-05).

Record type: Article

Abstract

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

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Published date: July 2006

Identifiers

Local EPrints ID: 419569
URI: http://eprints.soton.ac.uk/id/eprint/419569
ISSN: 0095-1137
PURE UUID: 033f5352-0496-4043-aef0-057522034c87
ORCID for G.J. Connett: ORCID iD orcid.org/0000-0003-1310-3239

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Date deposited: 13 Apr 2018 16:30
Last modified: 07 Oct 2020 02:20

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