Structural analysis of glycoproteins: building N-linked glycans with Coot
Structural analysis of glycoproteins: building N-linked glycans with Coot
Coot is a graphics application that is used to build or manipulate macromolecular
models; its particular forte is manipulation of the model at the residue level. The
model-building tools of Coot have been combined and extended to assist or
automate the building of N-linked glycans. The model is built by the addition of
monosaccharides, placed by variation of internal coordinates. The subsequent
model is refined by real-space refinement, which is stabilized with modified and
additional restraints. It is hoped that these enhanced building tools will help to
reduce building errors of N-linked glycans and improve our knowledge of the
structures of glycoproteins.
Emsley, Paul
4eaa0012-7fa6-43e7-82a1-0049231a8d1f
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
April 2018
Emsley, Paul
4eaa0012-7fa6-43e7-82a1-0049231a8d1f
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Emsley, Paul and Crispin, Max
(2018)
Structural analysis of glycoproteins: building N-linked glycans with Coot.
Acta Crystallographica Section D: Biological Crystallography, 74 (4).
(doi:10.1107/S2059798318005119).
Abstract
Coot is a graphics application that is used to build or manipulate macromolecular
models; its particular forte is manipulation of the model at the residue level. The
model-building tools of Coot have been combined and extended to assist or
automate the building of N-linked glycans. The model is built by the addition of
monosaccharides, placed by variation of internal coordinates. The subsequent
model is refined by real-space refinement, which is stabilized with modified and
additional restraints. It is hoped that these enhanced building tools will help to
reduce building errors of N-linked glycans and improve our knowledge of the
structures of glycoproteins.
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Accepted/In Press date: 29 March 2018
e-pub ahead of print date: 6 April 2018
Published date: April 2018
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Local EPrints ID: 419834
URI: http://eprints.soton.ac.uk/id/eprint/419834
ISSN: 0907-4449
PURE UUID: 10df4a32-c788-44d9-9be0-89aa975a4b1d
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Date deposited: 23 Apr 2018 16:30
Last modified: 16 Mar 2024 04:30
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Paul Emsley
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