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Using high resolution X-ray computed tomography to create an image based model of a lymph node

Using high resolution X-ray computed tomography to create an image based model of a lymph node
Using high resolution X-ray computed tomography to create an image based model of a lymph node
Lymph nodes are an important part of the immune system. They filter the lymphatic fluid as it is transported from the tissues before being returned to the blood stream. The fluid flow through the nodes influences the behaviour of the immune cells that gather within the nodes and the structure of the node itself. Measuring the fluid flow in lymph nodes experimentally is challenging due to their small size and fragility. In this paper, we present high resolution X-ray computed tomography images of a murine lymph node. The impact of the resulting visualized structures on fluid transport are investigated using an image based model. The high contrast between different structures within the lymph node provided by phase contrast X-ray computed tomography reconstruction results in images that, when related to the permeability of the lymph node tissue, suggest an increased fluid velocity through the interstitial channels in the lymph node tissue. Fluid taking a direct path from the afferent to the efferent lymphatic vessel, through the centre of the node, moved faster than the fluid that flowed around the periphery of the lymph node. This is a possible mechanism for particles being moved into the cortex.
Lymphatic System, Image based modelling, Porous media
0022-5193
73-82
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Zeller-Plumhoff, Berit
1a9d6525-3f1f-4a0b-89ba-e5042ceb37f4
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Ganapathisubramani, Bharathram
5e69099f-2f39-4fdd-8a85-3ac906827052
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Cooper, Laura
b8f4b942-cb87-4386-ab29-17bdd0f53ec1
Zeller-Plumhoff, Berit
1a9d6525-3f1f-4a0b-89ba-e5042ceb37f4
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Ganapathisubramani, Bharathram
5e69099f-2f39-4fdd-8a85-3ac906827052
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Cooper, Laura, Zeller-Plumhoff, Berit, Clough, Geraldine, Ganapathisubramani, Bharathram and Roose, Tiina (2018) Using high resolution X-ray computed tomography to create an image based model of a lymph node. Journal of Theoretical Biology, 449, 73-82. (doi:10.1016/j.jtbi.2018.04.021).

Record type: Article

Abstract

Lymph nodes are an important part of the immune system. They filter the lymphatic fluid as it is transported from the tissues before being returned to the blood stream. The fluid flow through the nodes influences the behaviour of the immune cells that gather within the nodes and the structure of the node itself. Measuring the fluid flow in lymph nodes experimentally is challenging due to their small size and fragility. In this paper, we present high resolution X-ray computed tomography images of a murine lymph node. The impact of the resulting visualized structures on fluid transport are investigated using an image based model. The high contrast between different structures within the lymph node provided by phase contrast X-ray computed tomography reconstruction results in images that, when related to the permeability of the lymph node tissue, suggest an increased fluid velocity through the interstitial channels in the lymph node tissue. Fluid taking a direct path from the afferent to the efferent lymphatic vessel, through the centre of the node, moved faster than the fluid that flowed around the periphery of the lymph node. This is a possible mechanism for particles being moved into the cortex.

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More information

Accepted/In Press date: 15 April 2018
e-pub ahead of print date: 17 April 2018
Published date: 14 July 2018
Keywords: Lymphatic System, Image based modelling, Porous media

Identifiers

Local EPrints ID: 419836
URI: http://eprints.soton.ac.uk/id/eprint/419836
ISSN: 0022-5193
PURE UUID: 750e1b3e-45e2-4b00-8d5a-ed94144f19e7
ORCID for Laura Cooper: ORCID iD orcid.org/0000-0003-0198-7591
ORCID for Geraldine Clough: ORCID iD orcid.org/0000-0002-6226-8964
ORCID for Bharathram Ganapathisubramani: ORCID iD orcid.org/0000-0001-9817-0486
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 23 Apr 2018 16:30
Last modified: 07 Oct 2020 06:28

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