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Harnessing human decellularized blood vessel matrices and cellular construct implants to promote bone healing in an ex vivo organotypic bone defect model

Harnessing human decellularized blood vessel matrices and cellular construct implants to promote bone healing in an ex vivo organotypic bone defect model
Harnessing human decellularized blood vessel matrices and cellular construct implants to promote bone healing in an ex vivo organotypic bone defect model
Decellularised matrices offer a beneficial substitute for biomimetic scaffolds in tissue engineering. The current study examines the potential of decellularised placental vessel sleeves (PVS) as a periosteal protective sleeve to enhance bone regeneration in embryonic day 18 chick femurs contained within the PVS and cultured organotypically over a 10 day period. The femurs are inserted into decellularised biocompatibility-tested PVS and maintained in an organotypic culture for a period of 10 days. In femurs containing decellularised PVS, a significant increase in bone volume (p<0.001) is evident demonstrated by micro-computed tomography (µCT) compared to femurs without PVS. Histological and immunohistological analysis revealed extensive integration of decellularised PVS with the bone periosteum, and enhanced conservation of bone architecture within the PVS. In addition the expression of Hypoxia inducible factor-1 alpha (HIF-1α), COL-II and proteoglycans were observed, indicating a possible repair mechanism via a cartilaginous stage of the bone tissue within the sleeve. The use of decellularised matrices like PVS offers a promising therapeutic strategy in surgical tissue replacement promoting biocompatibility and architecture of the tissue as well as a factor-rich niche environment with negligible immunogenicity.
2192-2659
1-32
Inglis, Stefanie
cb950bb9-8b89-41c0-b547-3206580d16b4
Schneider, Karl
47007025-ea38-4ff7-b5a6-2e9d6ee430f4
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Redl, H.
7164e93c-c525-4e94-90a5-dbbea536fc8d
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Inglis, Stefanie
cb950bb9-8b89-41c0-b547-3206580d16b4
Schneider, Karl
47007025-ea38-4ff7-b5a6-2e9d6ee430f4
Kanczler, Janos
eb8db9ff-a038-475f-9030-48eef2b0559c
Redl, H.
7164e93c-c525-4e94-90a5-dbbea536fc8d
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Inglis, Stefanie, Schneider, Karl, Kanczler, Janos, Redl, H. and Oreffo, Richard (2019) Harnessing human decellularized blood vessel matrices and cellular construct implants to promote bone healing in an ex vivo organotypic bone defect model. Advanced Healthcare Materials, 8 (9), 1-32, [1800088]. (doi:10.1002/adhm.201800088).

Record type: Article

Abstract

Decellularised matrices offer a beneficial substitute for biomimetic scaffolds in tissue engineering. The current study examines the potential of decellularised placental vessel sleeves (PVS) as a periosteal protective sleeve to enhance bone regeneration in embryonic day 18 chick femurs contained within the PVS and cultured organotypically over a 10 day period. The femurs are inserted into decellularised biocompatibility-tested PVS and maintained in an organotypic culture for a period of 10 days. In femurs containing decellularised PVS, a significant increase in bone volume (p<0.001) is evident demonstrated by micro-computed tomography (µCT) compared to femurs without PVS. Histological and immunohistological analysis revealed extensive integration of decellularised PVS with the bone periosteum, and enhanced conservation of bone architecture within the PVS. In addition the expression of Hypoxia inducible factor-1 alpha (HIF-1α), COL-II and proteoglycans were observed, indicating a possible repair mechanism via a cartilaginous stage of the bone tissue within the sleeve. The use of decellularised matrices like PVS offers a promising therapeutic strategy in surgical tissue replacement promoting biocompatibility and architecture of the tissue as well as a factor-rich niche environment with negligible immunogenicity.

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090418-Revised_manuscript-v2-AHM - Accepted Manuscript
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More information

Accepted/In Press date: 16 April 2018
e-pub ahead of print date: 14 May 2018
Published date: 9 May 2019

Identifiers

Local EPrints ID: 420267
URI: http://eprints.soton.ac.uk/id/eprint/420267
ISSN: 2192-2659
PURE UUID: 48b69b30-e373-422e-ba1f-1c5cdb4a2366
ORCID for Janos Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 03 May 2018 16:30
Last modified: 26 Nov 2021 06:45

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Contributors

Author: Stefanie Inglis
Author: Karl Schneider
Author: Janos Kanczler ORCID iD
Author: H. Redl
Author: Richard Oreffo ORCID iD

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