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Investigating the lymphatic drainage of the brain: essential skills and tools

Investigating the lymphatic drainage of the brain: essential skills and tools
Investigating the lymphatic drainage of the brain: essential skills and tools

In this chapter we describe in detail the surgical and imaging techniques employed for the study of the anatomical routes of drainage of cerebrospinal fluid (CSF) and interstitial fluid (ISF) from the brain. The types of tracers, sites of injection, and volumes injected are crucial. For example, when testing the drainage of ISF from the parenchyma, volumes larger than 0.5 μL result in spillage of ISF into the ventricular CSF.

Alzheimer Disease, Animals, Cisterna Magna, Extracellular Fluid, Fixatives, Fluorescent Dyes, Formaldehyde, Hippocampus, Humans, Immunohistochemistry, Injections, Intraventricular, Lymphatic Vessels, Mice, Microscopy, Fluorescence, Parenchymal Tissue, Perfusion, Polymers, Stereotaxic Techniques, Tissue Embedding, Xanthenes, Journal Article
343-365
Humana Press
Albargothy, Nazira J.
ec692e7e-550c-4914-a7c6-c8eb934b1a0f
Sharp, Matthew MacGregor
ec57c53a-a10a-4b8a-94fe-03eca85ab7c3
Gatherer, Maureen
bd7b1066-00cd-4673-82fd-2cec00f1ffc0
Morris, Alan
e70d7160-c9d9-4035-90b8-9006a1052551
Weller, Roy O.
4a501831-e38a-4d39-a125-d7141d6c667b
Hawkes, Cheryl
031a17ac-0931-4ff9-93cc-df8cb58e14f7
Carare, Roxana O.
0478c197-b0c1-4206-acae-54e88c8f21fa
Clausen, Björn E.
Laman, Jon D.
Albargothy, Nazira J.
ec692e7e-550c-4914-a7c6-c8eb934b1a0f
Sharp, Matthew MacGregor
ec57c53a-a10a-4b8a-94fe-03eca85ab7c3
Gatherer, Maureen
bd7b1066-00cd-4673-82fd-2cec00f1ffc0
Morris, Alan
e70d7160-c9d9-4035-90b8-9006a1052551
Weller, Roy O.
4a501831-e38a-4d39-a125-d7141d6c667b
Hawkes, Cheryl
031a17ac-0931-4ff9-93cc-df8cb58e14f7
Carare, Roxana O.
0478c197-b0c1-4206-acae-54e88c8f21fa
Clausen, Björn E.
Laman, Jon D.

Albargothy, Nazira J., Sharp, Matthew MacGregor, Gatherer, Maureen, Morris, Alan, Weller, Roy O., Hawkes, Cheryl and Carare, Roxana O. (2017) Investigating the lymphatic drainage of the brain: essential skills and tools. In, Clausen, Björn E. and Laman, Jon D. (eds.) Inflammation. (Methods in Molecular Biology, , (doi:10.1007/978-1-4939-6786-5_24), 1559) New York. Humana Press, pp. 343-365. (doi:10.1007/978-1-4939-6786-5_24).

Record type: Book Section

Abstract

In this chapter we describe in detail the surgical and imaging techniques employed for the study of the anatomical routes of drainage of cerebrospinal fluid (CSF) and interstitial fluid (ISF) from the brain. The types of tracers, sites of injection, and volumes injected are crucial. For example, when testing the drainage of ISF from the parenchyma, volumes larger than 0.5 μL result in spillage of ISF into the ventricular CSF.

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More information

e-pub ahead of print date: 7 January 2017
Published date: 2017
Keywords: Alzheimer Disease, Animals, Cisterna Magna, Extracellular Fluid, Fixatives, Fluorescent Dyes, Formaldehyde, Hippocampus, Humans, Immunohistochemistry, Injections, Intraventricular, Lymphatic Vessels, Mice, Microscopy, Fluorescence, Parenchymal Tissue, Perfusion, Polymers, Stereotaxic Techniques, Tissue Embedding, Xanthenes, Journal Article

Identifiers

Local EPrints ID: 420421
URI: https://eprints.soton.ac.uk/id/eprint/420421
PURE UUID: 808ad658-f376-4aca-83a6-59ea5a8ebda3
ORCID for Nazira J. Albargothy: ORCID iD orcid.org/0000-0003-1435-8612
ORCID for Matthew MacGregor Sharp: ORCID iD orcid.org/0000-0002-6623-5078

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Date deposited: 08 May 2018 16:30
Last modified: 03 Dec 2019 01:41

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Contributors

Author: Nazira J. Albargothy ORCID iD
Author: Matthew MacGregor Sharp ORCID iD
Author: Maureen Gatherer
Author: Alan Morris
Author: Roy O. Weller
Author: Cheryl Hawkes
Editor: Björn E. Clausen
Editor: Jon D. Laman

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