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The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain

The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain
The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain

Although there are no conventional lymphatic vessels in the brain, fluid and solutes drain along basement membranes (BMs) of cerebral capillaries and arteries towards the subarachnoid space and cervical lymph nodes. Convective influx/glymphatic entry of the cerebrospinal fluid (CSF) into the brain parenchyma occurs along the pial-glial BMs of arteries. This project tested the hypotheses that pial-glial BM of arteries are thicker in the midbrain, allowing more glymphatic entry of CSF. The in vivo MRI and PET images were obtained from a 4.2-year-old dog, whereas the post-mortem electron microscopy was performed in a 12-year-old dog. We demonstrated a significant increase in the thickness of the pial-glial BM in the midbrain compared with the same BM in different regions of the brain and an increase in the convective influx of fluid from the subarachnoid space. These results are highly significant for the intrathecal drug delivery into the brain, indicating that the midbrain is better equipped for convective influx/glymphatic entry of the CSF.

Animals, Arteries, Basement Membrane, Cerebrospinal Fluid, Dogs, Endothelium, Magnetic Resonance Imaging, Mesencephalon, Muscle, Smooth, Neuroglia, Pia Mater, Positron-Emission Tomography, Time Factors, Journal Article
0143-5221
2745-2752
Dobson, Howard
fae4e9b8-c96c-4d5a-b445-359fd9713d52
Sharp, Matthew MacGregor
ec57c53a-a10a-4b8a-94fe-03eca85ab7c3
Cumpsty, Richard
8cd1d339-5da7-459c-8c54-422434b19455
Criswell, Theodore P.
e13e2b82-4f3b-4ca0-a2ba-aebd4c3eddbf
Wellman, Tyler
0c7c0423-46e6-4be3-b2a2-916b6341e556
Finucane, Ciara
d50dd8c0-ac51-4e0e-94c5-6251187dbfba
Sullivan, Jenna M
47de2955-08d8-4495-a6b9-ced2ca3c2a45
Weller, Roy O.
4a501831-e38a-4d39-a125-d7141d6c667b
Verma, Ajay
5a207350-0de9-487a-be28-f9eb2f558140
Carare, Roxana O.
0478c197-b0c1-4206-acae-54e88c8f21fa
Dobson, Howard
fae4e9b8-c96c-4d5a-b445-359fd9713d52
Sharp, Matthew MacGregor
ec57c53a-a10a-4b8a-94fe-03eca85ab7c3
Cumpsty, Richard
8cd1d339-5da7-459c-8c54-422434b19455
Criswell, Theodore P.
e13e2b82-4f3b-4ca0-a2ba-aebd4c3eddbf
Wellman, Tyler
0c7c0423-46e6-4be3-b2a2-916b6341e556
Finucane, Ciara
d50dd8c0-ac51-4e0e-94c5-6251187dbfba
Sullivan, Jenna M
47de2955-08d8-4495-a6b9-ced2ca3c2a45
Weller, Roy O.
4a501831-e38a-4d39-a125-d7141d6c667b
Verma, Ajay
5a207350-0de9-487a-be28-f9eb2f558140
Carare, Roxana O.
0478c197-b0c1-4206-acae-54e88c8f21fa

Dobson, Howard, Sharp, Matthew MacGregor, Cumpsty, Richard, Criswell, Theodore P., Wellman, Tyler, Finucane, Ciara, Sullivan, Jenna M, Weller, Roy O., Verma, Ajay and Carare, Roxana O. (2017) The perivascular pathways for influx of cerebrospinal fluid are most efficient in the midbrain. Clinical Science, 131 (22), 2745-2752. (doi:10.1042/CS20171265).

Record type: Article

Abstract

Although there are no conventional lymphatic vessels in the brain, fluid and solutes drain along basement membranes (BMs) of cerebral capillaries and arteries towards the subarachnoid space and cervical lymph nodes. Convective influx/glymphatic entry of the cerebrospinal fluid (CSF) into the brain parenchyma occurs along the pial-glial BMs of arteries. This project tested the hypotheses that pial-glial BM of arteries are thicker in the midbrain, allowing more glymphatic entry of CSF. The in vivo MRI and PET images were obtained from a 4.2-year-old dog, whereas the post-mortem electron microscopy was performed in a 12-year-old dog. We demonstrated a significant increase in the thickness of the pial-glial BM in the midbrain compared with the same BM in different regions of the brain and an increase in the convective influx of fluid from the subarachnoid space. These results are highly significant for the intrathecal drug delivery into the brain, indicating that the midbrain is better equipped for convective influx/glymphatic entry of the CSF.

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More information

Accepted/In Press date: 9 October 2017
e-pub ahead of print date: 11 October 2017
Published date: 13 November 2017
Keywords: Animals, Arteries, Basement Membrane, Cerebrospinal Fluid, Dogs, Endothelium, Magnetic Resonance Imaging, Mesencephalon, Muscle, Smooth, Neuroglia, Pia Mater, Positron-Emission Tomography, Time Factors, Journal Article

Identifiers

Local EPrints ID: 420456
URI: http://eprints.soton.ac.uk/id/eprint/420456
ISSN: 0143-5221
PURE UUID: 767787a6-0488-4136-af9d-b325e5cd5bc0
ORCID for Matthew MacGregor Sharp: ORCID iD orcid.org/0000-0002-6623-5078
ORCID for Roxana O. Carare: ORCID iD orcid.org/0000-0001-6458-3776

Catalogue record

Date deposited: 08 May 2018 16:30
Last modified: 10 Nov 2021 03:25

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Contributors

Author: Howard Dobson
Author: Matthew MacGregor Sharp ORCID iD
Author: Richard Cumpsty
Author: Theodore P. Criswell
Author: Tyler Wellman
Author: Ciara Finucane
Author: Jenna M Sullivan
Author: Roy O. Weller
Author: Ajay Verma

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