The University of Southampton
University of Southampton Institutional Repository

Can peptides be orally-delivered in crustaceans? The case study of the Crustacean Hyperglycaemic Hormone in Procambarus clarkii

Can peptides be orally-delivered in crustaceans? The case study of the Crustacean Hyperglycaemic Hormone in Procambarus clarkii
Can peptides be orally-delivered in crustaceans? The case study of the Crustacean Hyperglycaemic Hormone in Procambarus clarkii
The oral administration of substances in crustaceans is a growing field of research, and is considered the most effective way to convey elements in their body instead injections or other invasive techniques. It presents huge potentialities, in fact the production of emulsions that preserves the substance to be conveyed until it reaches the target organ finds application in the development of new vaccines, in the convey of substances for aquaculture purposes and in the administration of stressors to contrast the spreading of invasive crustacean species. The red swamp crayfish (Procambarus clarkii, Girard 1852) is one of the most spread invasive species worldwide, but it is also considered among the most economically important freshwater crustacean species. For these reasons it is considered as a paradigmatic species.

The chemical synthesis of the D-Crustacean Hyperglycemic Hormone isoform (D-Phe-CHH) from P. clarkii is here presented, with its species-specificity, verified through the injection of D-Phe-CHH in both the red swamp crayfish and the threatened European species Austropotamobius italicus (Faxon, 1984). The D-Phe-CHH has been successfully oral-delivered in P. clarkii by using a water/oil/water microemulsion entrapped in a jellified matrix. After the administration of CHH, the glycaemia levels significantly increased in eyestalk-less animals at hour 6, and in intact crayfish at hour 5, from the ingestion.

These encouraging findings pave the way for the use of the double emulsion method to oral administer different peptides to both handle physiological aspects of P. clarkii life cycle for aquaculture purposes and to create species-specific peptides-based mix to hamper the spreading of invasive crayfish populations.
0044-8486
209-216
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Piazza, Federica
01b5e547-0569-480b-b1f3-a12ef9b4bee9
Cocchietto, Moreno
1dd7e6bb-dcf3-4ac0-b3f2-da0b0b5d08b7
Antcheva, Nikolinka
4c1a6760-b725-4ca8-9647-8e599ff377f8
Masiello, Domenico
f6f29c9a-a2a1-4c0e-b372-a83053de21cd
Franceschin, Andrea
e5f9ba8a-6388-41b8-bd17-9495b4c1a62b
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Bonzi, Lucrezia C.
a313299a-e184-49cc-ac0b-ed573deba5ac
Mosco, Alessandro
e8417712-bca7-4472-bb8c-4c492941b87d
Guarnaccia, Corrado
ce01c73d-cfe8-4565-83e1-9e0720d920cf
Sava, Gianni
5a21ab58-14b6-4b35-b988-91c4815b4d48
Giulianini, Piero Giulio
8d4881ad-49d3-408d-8327-c6a4f61d58b1
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Piazza, Federica
01b5e547-0569-480b-b1f3-a12ef9b4bee9
Cocchietto, Moreno
1dd7e6bb-dcf3-4ac0-b3f2-da0b0b5d08b7
Antcheva, Nikolinka
4c1a6760-b725-4ca8-9647-8e599ff377f8
Masiello, Domenico
f6f29c9a-a2a1-4c0e-b372-a83053de21cd
Franceschin, Andrea
e5f9ba8a-6388-41b8-bd17-9495b4c1a62b
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Bonzi, Lucrezia C.
a313299a-e184-49cc-ac0b-ed573deba5ac
Mosco, Alessandro
e8417712-bca7-4472-bb8c-4c492941b87d
Guarnaccia, Corrado
ce01c73d-cfe8-4565-83e1-9e0720d920cf
Sava, Gianni
5a21ab58-14b6-4b35-b988-91c4815b4d48
Giulianini, Piero Giulio
8d4881ad-49d3-408d-8327-c6a4f61d58b1

Manfrin, Chiara, Piazza, Federica, Cocchietto, Moreno, Antcheva, Nikolinka, Masiello, Domenico, Franceschin, Andrea, Peruzza, Luca, Bonzi, Lucrezia C., Mosco, Alessandro, Guarnaccia, Corrado, Sava, Gianni and Giulianini, Piero Giulio (2016) Can peptides be orally-delivered in crustaceans? The case study of the Crustacean Hyperglycaemic Hormone in Procambarus clarkii. Aquaculture, 463, 209-216. (doi:10.1016/j.aquaculture.2016.05.044).

Record type: Article

Abstract

The oral administration of substances in crustaceans is a growing field of research, and is considered the most effective way to convey elements in their body instead injections or other invasive techniques. It presents huge potentialities, in fact the production of emulsions that preserves the substance to be conveyed until it reaches the target organ finds application in the development of new vaccines, in the convey of substances for aquaculture purposes and in the administration of stressors to contrast the spreading of invasive crustacean species. The red swamp crayfish (Procambarus clarkii, Girard 1852) is one of the most spread invasive species worldwide, but it is also considered among the most economically important freshwater crustacean species. For these reasons it is considered as a paradigmatic species.

The chemical synthesis of the D-Crustacean Hyperglycemic Hormone isoform (D-Phe-CHH) from P. clarkii is here presented, with its species-specificity, verified through the injection of D-Phe-CHH in both the red swamp crayfish and the threatened European species Austropotamobius italicus (Faxon, 1984). The D-Phe-CHH has been successfully oral-delivered in P. clarkii by using a water/oil/water microemulsion entrapped in a jellified matrix. After the administration of CHH, the glycaemia levels significantly increased in eyestalk-less animals at hour 6, and in intact crayfish at hour 5, from the ingestion.

These encouraging findings pave the way for the use of the double emulsion method to oral administer different peptides to both handle physiological aspects of P. clarkii life cycle for aquaculture purposes and to create species-specific peptides-based mix to hamper the spreading of invasive crayfish populations.

This record has no associated files available for download.

More information

Accepted/In Press date: 31 May 2016
e-pub ahead of print date: 1 June 2016
Published date: 1 October 2016

Identifiers

Local EPrints ID: 420565
URI: http://eprints.soton.ac.uk/id/eprint/420565
ISSN: 0044-8486
PURE UUID: 2f1beca3-118e-412b-8873-71108a347d8e

Catalogue record

Date deposited: 10 May 2018 16:30
Last modified: 27 Apr 2022 04:26

Export record

Altmetrics

Contributors

Author: Chiara Manfrin
Author: Federica Piazza
Author: Moreno Cocchietto
Author: Nikolinka Antcheva
Author: Domenico Masiello
Author: Andrea Franceschin
Author: Luca Peruzza
Author: Lucrezia C. Bonzi
Author: Alessandro Mosco
Author: Corrado Guarnaccia
Author: Gianni Sava
Author: Piero Giulio Giulianini

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×