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Silencing two main isoforms of crustacean hyperglycemic hormone (CHH) induces compensatory expression of two CHH-like transcripts in the red swamp crayfish Procambarus clarkii

Silencing two main isoforms of crustacean hyperglycemic hormone (CHH) induces compensatory expression of two CHH-like transcripts in the red swamp crayfish Procambarus clarkii
Silencing two main isoforms of crustacean hyperglycemic hormone (CHH) induces compensatory expression of two CHH-like transcripts in the red swamp crayfish Procambarus clarkii
RNA interference has frequently been applied to modulate gene function in organisms. With the aim of creating new autocidal methods based on neuro-endocrine disruptors for invasive populations of Procambarus clarkii, we silenced the Crustacean Hyperglycemic Hormone (CHH) by injecting the corresponding dsRNA. CHH is a pleiotropic hormone that primarily regulates the mobilization of energy reserves and plays a pivotal role in stress responses. Here, we describe two experiments aimed at testing whether CHH silencing significantly alters important physiological aspects. The first experiment investigates the effects of CHH silencing at the glycemic and transcriptomic level in the eyestalk. The second experiment explores the long-term effects of CHH silencing and the effects on mortality and moulting rates. Osmotic deficits and mortality were recorded in specimens injected with CHH dsRNA, whilst controls were injected with GFP dsRNA. After 20 days, despite still silenced for CHH, individuals that survived recovered a strong hyperglycemic response after serotonin injection due to the compensatory effect of two peptides belonging to the crustacean neurohormone CHH protein family.
1824-307X
29-37
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Bonzi, Lucrezia
454164f6-b685-4bff-bba3-bc4d3dfa1eb5
Pallavicini, Alberto
250ed385-c46f-4011-92d8-34cb0039a627
Giulianini, Piero Giulio
8d4881ad-49d3-408d-8327-c6a4f61d58b1
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Bonzi, Lucrezia
454164f6-b685-4bff-bba3-bc4d3dfa1eb5
Pallavicini, Alberto
250ed385-c46f-4011-92d8-34cb0039a627
Giulianini, Piero Giulio
8d4881ad-49d3-408d-8327-c6a4f61d58b1

Manfrin, Chiara, Peruzza, Luca, Bonzi, Lucrezia, Pallavicini, Alberto and Giulianini, Piero Giulio (2015) Silencing two main isoforms of crustacean hyperglycemic hormone (CHH) induces compensatory expression of two CHH-like transcripts in the red swamp crayfish Procambarus clarkii. ISJ - Invertebrate Survival Journal, 12, 29-37.

Record type: Article

Abstract

RNA interference has frequently been applied to modulate gene function in organisms. With the aim of creating new autocidal methods based on neuro-endocrine disruptors for invasive populations of Procambarus clarkii, we silenced the Crustacean Hyperglycemic Hormone (CHH) by injecting the corresponding dsRNA. CHH is a pleiotropic hormone that primarily regulates the mobilization of energy reserves and plays a pivotal role in stress responses. Here, we describe two experiments aimed at testing whether CHH silencing significantly alters important physiological aspects. The first experiment investigates the effects of CHH silencing at the glycemic and transcriptomic level in the eyestalk. The second experiment explores the long-term effects of CHH silencing and the effects on mortality and moulting rates. Osmotic deficits and mortality were recorded in specimens injected with CHH dsRNA, whilst controls were injected with GFP dsRNA. After 20 days, despite still silenced for CHH, individuals that survived recovered a strong hyperglycemic response after serotonin injection due to the compensatory effect of two peptides belonging to the crustacean neurohormone CHH protein family.

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More information

Accepted/In Press date: 14 January 2015
Published date: 2015

Identifiers

Local EPrints ID: 420566
URI: https://eprints.soton.ac.uk/id/eprint/420566
ISSN: 1824-307X
PURE UUID: e61719ac-c4a6-4bfa-a626-17c7cda7e668

Catalogue record

Date deposited: 10 May 2018 16:30
Last modified: 13 Mar 2019 18:32

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