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Managing of Procambarus clarkii by X-ray sterilisation of males: Cytological damage to gonads

Managing of Procambarus clarkii by X-ray sterilisation of males: Cytological damage to gonads
Managing of Procambarus clarkii by X-ray sterilisation of males: Cytological damage to gonads
Procambarus clarkii is an invasive alien species spreading worldwide. It is therefore mandatory to find new methods to manage this species since traditional techniques are not sufficient for this purpose. The present study investigates gonad damage induced by different doses of ionising irradiation: 20, 40 and 60 Gy. Testis were analysed after 10 and 30 days by means of light, scanning and transmission electron microscopy. Control unirradiated testes present an acinar structure with a well-defined germinative cells maturation from the distal proliferative zone to the proximal stalk of the lobes whilst, in irradiated testes, induced apoptosis of germinative and accessory cells and a high level of vacuolisation inside the acini were identified, progressively increasing in accordance to Gy dosage and time after exposure. We determined the dose of 40 Gy as the best compromise: it causes an extensive damage to germinative tissues without affecting crayfish vitality, differing from 60 Gy. From an applicative point of view, this dose reduces the efforts, in terms of cost and time, for the application of SMRT.
0968-4328
32-40
Piazza, Federica
01b5e547-0569-480b-b1f3-a12ef9b4bee9
Aquiloni, Laura
e693f6e4-001e-411a-9b57-3c2cbf9ec004
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Simi, Sara
9ba17720-2aff-4cf5-8cec-a7a259372ce9
Marson, Lorena
f98783f8-2d6a-40bb-b6a8-c9e809775152
Edomi, Paolo
ef4a7b5d-d29c-4cf5-94a5-1ad76f8c2c70
Giulianini, Piero G.
4926ca3d-9edf-4589-b0d3-9aa341e85597
Piazza, Federica
01b5e547-0569-480b-b1f3-a12ef9b4bee9
Aquiloni, Laura
e693f6e4-001e-411a-9b57-3c2cbf9ec004
Peruzza, Luca
c01f028c-1fb4-44a4-83b9-f24698a9c9e5
Manfrin, Chiara
edc4fd1e-143d-4d3d-814f-2a4ec248145c
Simi, Sara
9ba17720-2aff-4cf5-8cec-a7a259372ce9
Marson, Lorena
f98783f8-2d6a-40bb-b6a8-c9e809775152
Edomi, Paolo
ef4a7b5d-d29c-4cf5-94a5-1ad76f8c2c70
Giulianini, Piero G.
4926ca3d-9edf-4589-b0d3-9aa341e85597

Piazza, Federica, Aquiloni, Laura, Peruzza, Luca, Manfrin, Chiara, Simi, Sara, Marson, Lorena, Edomi, Paolo and Giulianini, Piero G. (2015) Managing of Procambarus clarkii by X-ray sterilisation of males: Cytological damage to gonads. Micron, 77, 32-40. (doi:10.1016/j.micron.2015.05.016).

Record type: Article

Abstract

Procambarus clarkii is an invasive alien species spreading worldwide. It is therefore mandatory to find new methods to manage this species since traditional techniques are not sufficient for this purpose. The present study investigates gonad damage induced by different doses of ionising irradiation: 20, 40 and 60 Gy. Testis were analysed after 10 and 30 days by means of light, scanning and transmission electron microscopy. Control unirradiated testes present an acinar structure with a well-defined germinative cells maturation from the distal proliferative zone to the proximal stalk of the lobes whilst, in irradiated testes, induced apoptosis of germinative and accessory cells and a high level of vacuolisation inside the acini were identified, progressively increasing in accordance to Gy dosage and time after exposure. We determined the dose of 40 Gy as the best compromise: it causes an extensive damage to germinative tissues without affecting crayfish vitality, differing from 60 Gy. From an applicative point of view, this dose reduces the efforts, in terms of cost and time, for the application of SMRT.

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More information

Accepted/In Press date: 26 May 2015
e-pub ahead of print date: 30 May 2015
Published date: October 2015

Identifiers

Local EPrints ID: 420567
URI: https://eprints.soton.ac.uk/id/eprint/420567
ISSN: 0968-4328
PURE UUID: 9a338e1a-6f73-4798-bca0-ddc030dcfcf3

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Date deposited: 10 May 2018 16:30
Last modified: 13 Mar 2019 18:32

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Contributors

Author: Federica Piazza
Author: Laura Aquiloni
Author: Luca Peruzza
Author: Chiara Manfrin
Author: Sara Simi
Author: Lorena Marson
Author: Paolo Edomi
Author: Piero G. Giulianini

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