Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis
Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis
Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.
Antibiotics, Bacterial diversity, Pulmonary exacerbation, Relative abundance, T-RFLP
22-28
Daniels, T.W.V.
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Rogers, G.B.
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Stressmann, F.A.
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van der Gast, C.J.
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Bruce, K.D.
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Jones, G.R.
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Connett, G.J.
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Legg, J.P.
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Carroll, M.P.
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January 2013
Daniels, T.W.V.
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Rogers, G.B.
6c6aa9e9-3500-4777-9046-a77cb0c463d1
Stressmann, F.A.
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van der Gast, C.J.
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Bruce, K.D.
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Jones, G.R.
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Connett, G.J.
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Legg, J.P.
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Carroll, M.P.
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Daniels, T.W.V., Rogers, G.B., Stressmann, F.A., van der Gast, C.J., Bruce, K.D., Jones, G.R., Connett, G.J., Legg, J.P. and Carroll, M.P.
(2013)
Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis.
Journal of Cystic Fibrosis, 12 (1), .
(doi:10.1016/j.jcf.2012.05.008).
Abstract
Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.
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e-pub ahead of print date: 18 June 2012
Published date: January 2013
Keywords:
Antibiotics, Bacterial diversity, Pulmonary exacerbation, Relative abundance, T-RFLP
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Local EPrints ID: 420611
URI: http://eprints.soton.ac.uk/id/eprint/420611
ISSN: 1569-1993
PURE UUID: be1855ca-8deb-4dbd-804e-f622d6fb0d4b
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Date deposited: 10 May 2018 16:31
Last modified: 18 Mar 2024 03:46
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Contributors
Author:
T.W.V. Daniels
Author:
G.B. Rogers
Author:
F.A. Stressmann
Author:
C.J. van der Gast
Author:
K.D. Bruce
Author:
G.R. Jones
Author:
G.J. Connett
Author:
J.P. Legg
Author:
M.P. Carroll
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