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Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis
Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

Antibiotics, Bacterial diversity, Pulmonary exacerbation, Relative abundance, T-RFLP
1569-1993
22-28
Daniels, T.W.V.
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Rogers, G.B.
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Stressmann, F.A.
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van der Gast, C.J.
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Bruce, K.D.
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Jones, G.R.
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Connett, G.J.
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Legg, J.P.
8d6d78f4-3b30-4f4c-bcfa-9489a9fd386e
Carroll, M.P.
b836d262-6b07-4006-9c81-26653a26588b
Daniels, T.W.V.
d635a2fb-96a1-46ec-8cdf-8eb44a4bd0f5
Rogers, G.B.
6c6aa9e9-3500-4777-9046-a77cb0c463d1
Stressmann, F.A.
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van der Gast, C.J.
332ec7c1-c2c9-4d5e-a935-9e170980dfdb
Bruce, K.D.
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Jones, G.R.
ffe8ce54-bf80-45f0-a4d2-5b36e270d1a6
Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Legg, J.P.
8d6d78f4-3b30-4f4c-bcfa-9489a9fd386e
Carroll, M.P.
b836d262-6b07-4006-9c81-26653a26588b

Daniels, T.W.V., Rogers, G.B., Stressmann, F.A., van der Gast, C.J., Bruce, K.D., Jones, G.R., Connett, G.J., Legg, J.P. and Carroll, M.P. (2013) Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis. Journal of Cystic Fibrosis, 12 (1), 22-28. (doi:10.1016/j.jcf.2012.05.008).

Record type: Article

Abstract

Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

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e-pub ahead of print date: 18 June 2012
Published date: January 2013
Keywords: Antibiotics, Bacterial diversity, Pulmonary exacerbation, Relative abundance, T-RFLP

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Local EPrints ID: 420611
URI: http://eprints.soton.ac.uk/id/eprint/420611
ISSN: 1569-1993
PURE UUID: be1855ca-8deb-4dbd-804e-f622d6fb0d4b
ORCID for G.J. Connett: ORCID iD orcid.org/0000-0003-1310-3239

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Date deposited: 10 May 2018 16:31
Last modified: 26 Nov 2019 01:23

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Contributors

Author: T.W.V. Daniels
Author: G.B. Rogers
Author: F.A. Stressmann
Author: C.J. van der Gast
Author: K.D. Bruce
Author: G.R. Jones
Author: G.J. Connett ORCID iD
Author: J.P. Legg
Author: M.P. Carroll

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