The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming and biological effects

Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming and biological effects
Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming and biological effects
The study of the effects of ultrasound-induced acoustic cavitation on biological structures is an active field in biomedical research. Of particular interest for therapeutic applications is the ability of oscillating microbubbles to promote both cellular and tissue membrane permeabilisation and to improve the distribution of therapeutic agents in tissue through extravasation and convective transport. The mechanisms that underpin the interaction between cavitating agents and tissues are, however, still poorly understood. One challenge is the practical difficulty involved in performing optical microscopy and acoustic emissions monitoring simultaneously in a biologically compatible environment. Here we present and characterise a microfluidic layered acoustic resonator (μLAR) developed for simultaneous ultrasound exposure, acoustic emissions monitoring and microscopy of biological samples. The μLAR facilitates in vitro ultrasound experiments in which measurements of microbubble dynamics, microstreaming velocity fields, acoustic emissions and cell-microbubble interactions can be performed simultaneously. The device and analyses presented provide a means of performing mechanistic in vitro studies that may benefit the design of predictable and effective cavitation-based ultrasound treatments.
1932-1058
1-14
Pereno, Valerio
3106035d-e7d9-4af8-af59-dd5091532ea7
Aron, Miles
4d9c7843-bbe5-4a5d-975f-ea58a09fc621
Vince, Oliver
47570998-4408-4539-b6db-860768d39fc9
Mannaris, Christophoros
ec9c3302-0a0a-4953-840b-26ff09b4e633
Seth, Anjali
92f275f5-ebf6-4e76-9c16-d8438f765ca2
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Lajoinie, Guillaume
6f33c02e-166a-46dd-a772-347bd4628a2a
Versluis, Michel
0f3d7824-c8f1-47b1-a14e-46453b5ec901
Coussios, Constantin
55b78f4c-f241-4676-87d5-9ea5fb295f4f
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6
Pereno, Valerio
3106035d-e7d9-4af8-af59-dd5091532ea7
Aron, Miles
4d9c7843-bbe5-4a5d-975f-ea58a09fc621
Vince, Oliver
47570998-4408-4539-b6db-860768d39fc9
Mannaris, Christophoros
ec9c3302-0a0a-4953-840b-26ff09b4e633
Seth, Anjali
92f275f5-ebf6-4e76-9c16-d8438f765ca2
de Saint Victor, Marie
b558a637-b7b6-4b5f-ad84-1a93f74a425e
Lajoinie, Guillaume
6f33c02e-166a-46dd-a772-347bd4628a2a
Versluis, Michel
0f3d7824-c8f1-47b1-a14e-46453b5ec901
Coussios, Constantin
55b78f4c-f241-4676-87d5-9ea5fb295f4f
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Stride, Eleanor
c0143e95-81fa-47c8-b9bc-5b4fc319bba6

Pereno, Valerio, Aron, Miles, Vince, Oliver, Mannaris, Christophoros, Seth, Anjali, de Saint Victor, Marie, Lajoinie, Guillaume, Versluis, Michel, Coussios, Constantin, Carugo, Dario and Stride, Eleanor (2018) Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming and biological effects. Biomicrofluidics, 12 (3), 1-14, [034109]. (doi:10.1063/1.5023729).

Record type: Article

Abstract

The study of the effects of ultrasound-induced acoustic cavitation on biological structures is an active field in biomedical research. Of particular interest for therapeutic applications is the ability of oscillating microbubbles to promote both cellular and tissue membrane permeabilisation and to improve the distribution of therapeutic agents in tissue through extravasation and convective transport. The mechanisms that underpin the interaction between cavitating agents and tissues are, however, still poorly understood. One challenge is the practical difficulty involved in performing optical microscopy and acoustic emissions monitoring simultaneously in a biologically compatible environment. Here we present and characterise a microfluidic layered acoustic resonator (μLAR) developed for simultaneous ultrasound exposure, acoustic emissions monitoring and microscopy of biological samples. The μLAR facilitates in vitro ultrasound experiments in which measurements of microbubble dynamics, microstreaming velocity fields, acoustic emissions and cell-microbubble interactions can be performed simultaneously. The device and analyses presented provide a means of performing mechanistic in vitro studies that may benefit the design of predictable and effective cavitation-based ultrasound treatments.

Text
pdf_archiveBIOMGBvol_12iss_3034109_1_am - Accepted Manuscript
Download (4MB)
Text
Biomicrofluidics-Accepted-2018 - Accepted Manuscript
Restricted to Repository staff only
Request a copy
Text
1.5023729 - Version of Record
Available under License Creative Commons Attribution.
Download (2MB)

More information

Accepted/In Press date: 9 May 2018
e-pub ahead of print date: 30 May 2018

Identifiers

Local EPrints ID: 420911
URI: http://eprints.soton.ac.uk/id/eprint/420911
ISSN: 1932-1058
PURE UUID: 3867b064-d17e-4d9f-b2da-013703e1d391

Catalogue record

Date deposited: 18 May 2018 16:30
Last modified: 26 Nov 2021 07:07

Export record

Altmetrics

Contributors

Author: Valerio Pereno
Author: Miles Aron
Author: Oliver Vince
Author: Christophoros Mannaris
Author: Anjali Seth
Author: Marie de Saint Victor
Author: Guillaume Lajoinie
Author: Michel Versluis
Author: Constantin Coussios
Author: Dario Carugo
Author: Eleanor Stride

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×