Effect of dietary fat on the in vitro hepatotoxicity of paracetamol
Effect of dietary fat on the in vitro hepatotoxicity of paracetamol
In the present study, we have examined the effect of dietary fat on paracetamol-induced liver injury in an in vitro rat liver slice model. Rats were fed, for 7-10 days, diets containing either butter or polyunsaturated vegetable margarine, two fat sources commonly consumed in the human diet. Liver slices were then exposed to paracetamol for 2 hr and further incubated for 4 hr without paracetamol. Cell damage in the slices was quantified at 6 hr by measuring leakage of lactate dehydrogenase, increase in water content and potassium loss. Covalent binding of radioactive paracetamol to liver and the membrane fatty acid composition of the liver were also measured. Liver slices from rats fed butter diets were significantly more sensitive to the toxic effects of paracetamol than those from margarine fed rats. The membrane lipid composition of the livers also reflected the differing fatty acid content of the two diets.
Acetaminophen, Animals, Dietary Fats, Dietary Fats, Unsaturated, Fatty Acids, L-Lactate Dehydrogenase, Liver, Male, Microsomes, Liver, Potassium, Rats, Rats, Wistar, Water, Comparative Study, Journal Article
1303-6
McDanell, R.E.
cb169a49-bc42-465c-899a-40a6b1f7a985
Beales, D.
c52890fb-cbf3-408b-a9a6-37bcf4f3cdc7
Henderson, L.
3f24b108-2a16-459c-b515-fa707b9a52bf
Sethi, J.K.
923f1a81-91e4-46cd-8853-bb4a979f5a85
6 October 1992
McDanell, R.E.
cb169a49-bc42-465c-899a-40a6b1f7a985
Beales, D.
c52890fb-cbf3-408b-a9a6-37bcf4f3cdc7
Henderson, L.
3f24b108-2a16-459c-b515-fa707b9a52bf
Sethi, J.K.
923f1a81-91e4-46cd-8853-bb4a979f5a85
McDanell, R.E., Beales, D., Henderson, L. and Sethi, J.K.
(1992)
Effect of dietary fat on the in vitro hepatotoxicity of paracetamol.
Biochemical Pharmacology, 44 (7), .
(doi:10.1016/0006-2952(92)90530-V).
Abstract
In the present study, we have examined the effect of dietary fat on paracetamol-induced liver injury in an in vitro rat liver slice model. Rats were fed, for 7-10 days, diets containing either butter or polyunsaturated vegetable margarine, two fat sources commonly consumed in the human diet. Liver slices were then exposed to paracetamol for 2 hr and further incubated for 4 hr without paracetamol. Cell damage in the slices was quantified at 6 hr by measuring leakage of lactate dehydrogenase, increase in water content and potassium loss. Covalent binding of radioactive paracetamol to liver and the membrane fatty acid composition of the liver were also measured. Liver slices from rats fed butter diets were significantly more sensitive to the toxic effects of paracetamol than those from margarine fed rats. The membrane lipid composition of the livers also reflected the differing fatty acid content of the two diets.
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Accepted/In Press date: 14 July 1992
Published date: 6 October 1992
Keywords:
Acetaminophen, Animals, Dietary Fats, Dietary Fats, Unsaturated, Fatty Acids, L-Lactate Dehydrogenase, Liver, Male, Microsomes, Liver, Potassium, Rats, Rats, Wistar, Water, Comparative Study, Journal Article
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Local EPrints ID: 421032
URI: http://eprints.soton.ac.uk/id/eprint/421032
ISSN: 0006-2952
PURE UUID: 2d527d18-46ea-4f6b-9071-cc2c2a0ef48e
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Date deposited: 21 May 2018 16:30
Last modified: 16 Mar 2024 04:31
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Author:
R.E. McDanell
Author:
D. Beales
Author:
L. Henderson
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