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Nitric oxide releases intracellular zinc from prokaryotic metallothionein in Escherichia coli

Nitric oxide releases intracellular zinc from prokaryotic metallothionein in Escherichia coli
Nitric oxide releases intracellular zinc from prokaryotic metallothionein in Escherichia coli

Nitric oxide (NO) has a broad spectrum of signalling and regulatory functions and multiple molecular targets. Recently, the intrabacterial toxicity of NO and mechanisms for NO resistance have been intensively investigated. Here we report for the first time that NO elicits release of zinc from a bacterial protein. Using the zinc-responsive expression of zntA (encoding a Zn-exporting P-type ATPase) fused to lacZ, i.e. Phi(zntA-lacZ), to monitor intracellular zinc, and SmtA (the Synechococcus metallothionein) as zinc store, we have shown that the NO donors NOC-5 and NOC-7 elicit zinc ejection. No increase in Phi(zntA-lacZ) activity was observed in a zntR mutant, indicating the specificity of the zntA promoter response to zinc ions.

Adenosine Triphosphatases, Escherichia coli, Metallothionein, Mutation, Nitric Oxide, Promoter Regions, Genetic, Zinc, Journal Article, Research Support, Non-U.S. Gov't
0378-1097
121-126
Binet, Marie R B
23472b8a-cf0b-4271-ba32-273200f2d0c5
Cruz-Ramos, Hugo
743673a8-a438-44cb-873e-c09373302cdd
Laver, Jay
b2996398-2ccf-40f0-92b8-f338f3de796b
Hughes, Martin N
b3cad4ed-70d1-4f48-9f69-a727fe884710
Poole, Robert K
5f1f3b79-cf45-4ae6-89cd-e4259c03ae56
Binet, Marie R B
23472b8a-cf0b-4271-ba32-273200f2d0c5
Cruz-Ramos, Hugo
743673a8-a438-44cb-873e-c09373302cdd
Laver, Jay
b2996398-2ccf-40f0-92b8-f338f3de796b
Hughes, Martin N
b3cad4ed-70d1-4f48-9f69-a727fe884710
Poole, Robert K
5f1f3b79-cf45-4ae6-89cd-e4259c03ae56

Binet, Marie R B, Cruz-Ramos, Hugo, Laver, Jay, Hughes, Martin N and Poole, Robert K (2002) Nitric oxide releases intracellular zinc from prokaryotic metallothionein in Escherichia coli. FEMS Microbiology Letters, 213 (1), 121-126. (doi:10.1111/j.1574-6968.2002.tb11295.x).

Record type: Article

Abstract

Nitric oxide (NO) has a broad spectrum of signalling and regulatory functions and multiple molecular targets. Recently, the intrabacterial toxicity of NO and mechanisms for NO resistance have been intensively investigated. Here we report for the first time that NO elicits release of zinc from a bacterial protein. Using the zinc-responsive expression of zntA (encoding a Zn-exporting P-type ATPase) fused to lacZ, i.e. Phi(zntA-lacZ), to monitor intracellular zinc, and SmtA (the Synechococcus metallothionein) as zinc store, we have shown that the NO donors NOC-5 and NOC-7 elicit zinc ejection. No increase in Phi(zntA-lacZ) activity was observed in a zntR mutant, indicating the specificity of the zntA promoter response to zinc ions.

Full text not available from this repository.

More information

e-pub ahead of print date: 9 January 2002
Published date: 16 July 2002
Keywords: Adenosine Triphosphatases, Escherichia coli, Metallothionein, Mutation, Nitric Oxide, Promoter Regions, Genetic, Zinc, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 421237
URI: http://eprints.soton.ac.uk/id/eprint/421237
ISSN: 0378-1097
PURE UUID: ac0f2dc8-2bbd-42cb-8472-df876c413c98
ORCID for Jay Laver: ORCID iD orcid.org/0000-0003-3314-5989

Catalogue record

Date deposited: 25 May 2018 16:30
Last modified: 18 Feb 2021 17:20

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