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Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces

Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces
Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces
Release of metal ions from metal-based surfaces has been considered one of the main drivers of their antimicrobial activity. Here we describe a method that enables parallel assessment of metal ion release from solid metallic surfaces and antimicrobial efficacy of these surfaces in a short time period. The protocol involves placement of a small volume of bioluminescent bacteria onto the tested surface and direct measurement of bioluminescence at various time points. In this study, two recombinant Escherichia coli strains, one expressing bioluminescence constitutively and applicable for general antimicrobial testing, and the other induced by Cu ions, were selected. Decrease in bioluminescence of constitutive E. coli on the surfaces showed a good correlation with the decrease in bacterial viability. Response of Cu-inducible E. coli showed a correlation with Cu content in the tested surfaces but not with Cu dissolution suggesting the role of direct bacteria-surface contact in Cu ion-driven antibacterial effects. In summary, the presented protocol enables the analysis of microbial toxicity and bioavailability of surface-released metal ions directly on solid surfaces within 30-60 min. Although optimized for copper and copper alloy surfaces and E. coli, the method can be extended to other types of metallic surfaces and bacterial strains.
copper and copper alloys, antimicrobial, sensor, Escherichia coli
2045-2322
1-12
Rosenberg, Merilin
49247e8b-d65d-4318-adf7-8c0496e59f76
Vija, Heiki
75de7572-0432-419e-84be-9ddb0fa6ad28
Kahru, Anne
2c9e28a6-bc24-4e02-bfdd-d329914a7747
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Ivask, Angela
2a60121a-01d4-496f-9051-d194e2fa2627
Rosenberg, Merilin
49247e8b-d65d-4318-adf7-8c0496e59f76
Vija, Heiki
75de7572-0432-419e-84be-9ddb0fa6ad28
Kahru, Anne
2c9e28a6-bc24-4e02-bfdd-d329914a7747
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Ivask, Angela
2a60121a-01d4-496f-9051-d194e2fa2627

Rosenberg, Merilin, Vija, Heiki, Kahru, Anne, Keevil, Charles and Ivask, Angela (2018) Rapid in situ assessment of Cu-ion mediated effects and antibacterial efficacy of copper surfaces. Scientific Reports, 8, 1-12, [8172]. (doi:10.1038/s41598-018-26391-8).

Record type: Article

Abstract

Release of metal ions from metal-based surfaces has been considered one of the main drivers of their antimicrobial activity. Here we describe a method that enables parallel assessment of metal ion release from solid metallic surfaces and antimicrobial efficacy of these surfaces in a short time period. The protocol involves placement of a small volume of bioluminescent bacteria onto the tested surface and direct measurement of bioluminescence at various time points. In this study, two recombinant Escherichia coli strains, one expressing bioluminescence constitutively and applicable for general antimicrobial testing, and the other induced by Cu ions, were selected. Decrease in bioluminescence of constitutive E. coli on the surfaces showed a good correlation with the decrease in bacterial viability. Response of Cu-inducible E. coli showed a correlation with Cu content in the tested surfaces but not with Cu dissolution suggesting the role of direct bacteria-surface contact in Cu ion-driven antibacterial effects. In summary, the presented protocol enables the analysis of microbial toxicity and bioavailability of surface-released metal ions directly on solid surfaces within 30-60 min. Although optimized for copper and copper alloy surfaces and E. coli, the method can be extended to other types of metallic surfaces and bacterial strains.

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193586_2_merged_1525294594 - Accepted Manuscript
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More information

Accepted/In Press date: 11 May 2018
e-pub ahead of print date: 25 May 2018
Keywords: copper and copper alloys, antimicrobial, sensor, Escherichia coli

Identifiers

Local EPrints ID: 421240
URI: http://eprints.soton.ac.uk/id/eprint/421240
ISSN: 2045-2322
PURE UUID: 319729eb-73da-4068-aa9a-ac7a4f2b1ed2
ORCID for Charles Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 25 May 2018 16:30
Last modified: 17 Dec 2019 05:26

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