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In situ vital staining for chasing the galatheid crab Shinkaia crosnieri on deep-sea floor

In situ vital staining for chasing the galatheid crab Shinkaia crosnieri on deep-sea floor
In situ vital staining for chasing the galatheid crab Shinkaia crosnieri on deep-sea floor
Shinkaia crosnieri, a galatheid crab, has ectosymbiotic bacteria on its ventral setae, and forms very dense crowds in hydrothermally active regions and seep areas. They feed on the symbiotic bacteria and do not chase other animals for predation. To study how they move and behave in jostling crowds, we developed a vital staining to mark their individuals and trace them by using a camera on a remotely operated vehicle (ROV). Among the various dyes examined, Coomasie Brilliant Blue R250 (CBB) stained the galatheid crab the darkest, and its color lasted for more than 5 months in the laboratory at 4–5°C. The ventral setae were strongly stained, while the dorsal shell was weakly stained. The stained galatheid crab survived for more than 8 months. For the in situ staining of S. crosnieri at the Iheya North hydrothermal field in the Okinawa Trough, Japan, we applied a dye solution mixture (20 L) containing CBB and Acid Blue 161 to the galatheid crab population through a funnel equipped on the ROV Hyper-Dolphin. After staining for approximately 5 minutes, more than 18 individuals of S. crosnieri were dyed blue. They were disturbed by the staining process but seemed to be unharmed. The dyed galatheid crabs were identified by the ROV one and two days post staining. They seemed to remain at the place where they were stained.
The present vital-staining marking method may present a new way to analyze the behavior and changing habitable range of deep-sea animals like S. crosnieri, and may give us a deeper insight into how these animals behave in a very dense population and explore newer habitats.
87-97
Maruyama, Tadashi
356405a6-17aa-4024-9716-910bb56de9f6
Takahashi, Tomoko
937057f6-8e83-4a7f-b11f-b549c94afdf6
Watsuji, Tomo-o
683c9b87-9e4c-42e0-ab74-279a648e9ac5
Kayama-Watanabe, Hiromi
6b9a99ca-a827-445a-abd7-d7df423a85c3
Nagai, Yukiko
a837897a-19f1-4ab8-8c26-13b079318d09
Fujiwara, Yoshihiro
b60f0d50-9167-4468-9e08-431508dfafe6
Toyofuku, Takashi
62c93efe-9278-4fc6-b07b-5d64432c22a9
Nemoto, Suguru
091ba060-87ec-4116-9396-bfe622aab7a4
Koyano, Yuka
6f7be393-4724-4561-9c0b-41842b38ea9e
Thornton, Blair
8293beb5-c083-47e3-b5f0-d9c3cee14be9
Maruyama, Tadashi
356405a6-17aa-4024-9716-910bb56de9f6
Takahashi, Tomoko
937057f6-8e83-4a7f-b11f-b549c94afdf6
Watsuji, Tomo-o
683c9b87-9e4c-42e0-ab74-279a648e9ac5
Kayama-Watanabe, Hiromi
6b9a99ca-a827-445a-abd7-d7df423a85c3
Nagai, Yukiko
a837897a-19f1-4ab8-8c26-13b079318d09
Fujiwara, Yoshihiro
b60f0d50-9167-4468-9e08-431508dfafe6
Toyofuku, Takashi
62c93efe-9278-4fc6-b07b-5d64432c22a9
Nemoto, Suguru
091ba060-87ec-4116-9396-bfe622aab7a4
Koyano, Yuka
6f7be393-4724-4561-9c0b-41842b38ea9e
Thornton, Blair
8293beb5-c083-47e3-b5f0-d9c3cee14be9

Maruyama, Tadashi, Takahashi, Tomoko, Watsuji, Tomo-o, Kayama-Watanabe, Hiromi, Nagai, Yukiko, Fujiwara, Yoshihiro, Toyofuku, Takashi, Nemoto, Suguru, Koyano, Yuka and Thornton, Blair (2018) In situ vital staining for chasing the galatheid crab Shinkaia crosnieri on deep-sea floor. JAMSTEC Report of Research and Development, 27, 87-97. (doi:10.5918/jamstecr.27.87).

Record type: Article

Abstract

Shinkaia crosnieri, a galatheid crab, has ectosymbiotic bacteria on its ventral setae, and forms very dense crowds in hydrothermally active regions and seep areas. They feed on the symbiotic bacteria and do not chase other animals for predation. To study how they move and behave in jostling crowds, we developed a vital staining to mark their individuals and trace them by using a camera on a remotely operated vehicle (ROV). Among the various dyes examined, Coomasie Brilliant Blue R250 (CBB) stained the galatheid crab the darkest, and its color lasted for more than 5 months in the laboratory at 4–5°C. The ventral setae were strongly stained, while the dorsal shell was weakly stained. The stained galatheid crab survived for more than 8 months. For the in situ staining of S. crosnieri at the Iheya North hydrothermal field in the Okinawa Trough, Japan, we applied a dye solution mixture (20 L) containing CBB and Acid Blue 161 to the galatheid crab population through a funnel equipped on the ROV Hyper-Dolphin. After staining for approximately 5 minutes, more than 18 individuals of S. crosnieri were dyed blue. They were disturbed by the staining process but seemed to be unharmed. The dyed galatheid crabs were identified by the ROV one and two days post staining. They seemed to remain at the place where they were stained.
The present vital-staining marking method may present a new way to analyze the behavior and changing habitable range of deep-sea animals like S. crosnieri, and may give us a deeper insight into how these animals behave in a very dense population and explore newer habitats.

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More information

Accepted/In Press date: 25 May 2018
e-pub ahead of print date: 29 May 2018
Published date: September 2018

Identifiers

Local EPrints ID: 421284
URI: http://eprints.soton.ac.uk/id/eprint/421284
PURE UUID: ddafba7e-fe2c-453f-aedc-e8284aa8e5b0

Catalogue record

Date deposited: 31 May 2018 16:30
Last modified: 06 Nov 2018 17:30

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