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The distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies?

The distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies?
The distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies?
AIMS:To identify the presence and geographical distribution of mast cell (MC) subtypes: MCT (tryptase positive-chymase negative) and MCTC (tryptase positive-chymase positive) in bladder tissue. METHODS:Bladder tissue was obtained from patients with painful bladder syndrome/interstitial cystitis (n=14) and normal histology from University Hospital Southampton tissue bank. Sequential tissue slices were immunohistochemically stained for MC subtypes using anti-MC tryptase (for MCT and MCTC) and anti-MC chymase (for MCTC). Stained sections were photographed, and positively stained MCs were quantified using ImageJ. Data were analysed using descriptive statistics and individual paired t-tests. RESULTS:There was a significant difference in the density of MCs between each layer of the disease bladder, with the greatest accumulation within the detrusor (p<0.001). There was a significant increase in MCTC subtype in the lamina (p=0.009) in painful bladder syndrome/interstitial cystitis. CONCLUSIONS:Our results suggest that mastocytosis is present within all layers of disease bladder, especially the muscle layer. The varying increase in MC subtypes in the lamina and mucosa may explain the variability in painful bladder syndrome/interstitial cystitis symptoms. A high influx of MCTC in the mucosa of individuals who also had ulceration noted within their diagnostic notes may be of the Hunner's ulcer subclassification. These findings suggest a relationship between the pathogenesis of MC subtypes and the clinical presentation of painful bladder syndrome/interstitial cystitis. A cohort study would further elucidate the diagnostic and/or therapeutic potential of MCs in patients with painful bladder syndrome/interstitial cystitis.
0021-9746
1-5
Malik, Shabana
90d9ab88-16ad-47ea-b328-7759acd68ada
Birch, Brian R.
536ee8d2-9cf9-4412-a29b-d2267fa9d765
Voegeli, David
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Fader, Miranda
c318f942-2ddb-462a-9183-8b678faf7277
Foria, Vipul
02f133f1-ae11-433c-b18d-64efb40258a7
Cooper, Alan J.
0fd55ea3-e62b-4a06-bcc6-332b51cb22e4
Walls, Andrew
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
El Wilid, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21
Malik, Shabana
90d9ab88-16ad-47ea-b328-7759acd68ada
Birch, Brian R.
536ee8d2-9cf9-4412-a29b-d2267fa9d765
Voegeli, David
e6f5d112-55b0-40c1-a6ad-8929a2d84a10
Fader, Miranda
c318f942-2ddb-462a-9183-8b678faf7277
Foria, Vipul
02f133f1-ae11-433c-b18d-64efb40258a7
Cooper, Alan J.
0fd55ea3-e62b-4a06-bcc6-332b51cb22e4
Walls, Andrew
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
El Wilid, Bashir
e7c59131-82ad-4a14-a227-7370e91e3f21

Malik, Shabana, Birch, Brian R., Voegeli, David, Fader, Miranda, Foria, Vipul, Cooper, Alan J., Walls, Andrew and El Wilid, Bashir (2018) The distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies? Journal of Clinical Pathology, 1-5. (doi:10.1136/jclinpath-2017-204881).

Record type: Article

Abstract

AIMS:To identify the presence and geographical distribution of mast cell (MC) subtypes: MCT (tryptase positive-chymase negative) and MCTC (tryptase positive-chymase positive) in bladder tissue. METHODS:Bladder tissue was obtained from patients with painful bladder syndrome/interstitial cystitis (n=14) and normal histology from University Hospital Southampton tissue bank. Sequential tissue slices were immunohistochemically stained for MC subtypes using anti-MC tryptase (for MCT and MCTC) and anti-MC chymase (for MCTC). Stained sections were photographed, and positively stained MCs were quantified using ImageJ. Data were analysed using descriptive statistics and individual paired t-tests. RESULTS:There was a significant difference in the density of MCs between each layer of the disease bladder, with the greatest accumulation within the detrusor (p<0.001). There was a significant increase in MCTC subtype in the lamina (p=0.009) in painful bladder syndrome/interstitial cystitis. CONCLUSIONS:Our results suggest that mastocytosis is present within all layers of disease bladder, especially the muscle layer. The varying increase in MC subtypes in the lamina and mucosa may explain the variability in painful bladder syndrome/interstitial cystitis symptoms. A high influx of MCTC in the mucosa of individuals who also had ulceration noted within their diagnostic notes may be of the Hunner's ulcer subclassification. These findings suggest a relationship between the pathogenesis of MC subtypes and the clinical presentation of painful bladder syndrome/interstitial cystitis. A cohort study would further elucidate the diagnostic and/or therapeutic potential of MCs in patients with painful bladder syndrome/interstitial cystitis.

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Accepted/In Press date: 10 April 2018
e-pub ahead of print date: 15 May 2018

Identifiers

Local EPrints ID: 421301
URI: https://eprints.soton.ac.uk/id/eprint/421301
ISSN: 0021-9746
PURE UUID: ad9af927-c0f8-4280-bd2f-6719c3d7d4c0
ORCID for David Voegeli: ORCID iD orcid.org/0000-0003-3457-7177

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Date deposited: 31 May 2018 16:30
Last modified: 13 Mar 2019 18:38

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