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Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass

Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass
Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass
Background & aims

Roux-en-Y gastric bypass (RYGB) limits food ingestion and may alter the intestinal expression of genes involved in the endogenous synthesis of polyunsaturated fatty acids (PUFAs). These changes may decrease the systemic availability of bioactive PUFAs after RYGB.

Aim

To study the impact of RYGB on the dietary ingestion and plasma concentration of PUFAs and on the intestinal expression of genes involved in their endogenous biosynthesis in severely obese women with type 2 diabetes.

Methods

Before, and 3 and 12 months after RYGB, obese women (n = 20) self-reported a seven-day dietary record, answered a food frequency query and provided plasma samples for alpha-linolenic (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acid assessment by gas chromatography. Intestinal biopsies(duodenum, jejunum and ileum) were collected through double-balloon endoscopybefore and 3 months after RYGB for gene expression analysis by microarray (Human GeneChip 1.0 ST array) and RT-qPCR validation.

Results

Compared to the preoperative period, patients had decreased intakes of PUFAs, fish and soybean oil (p < 0.05) and lower plasma concentrations of ALA and EPA (p < 0.001) 3 and 12 months after RYGB. FADS1 gene expression was lower in duodenum (RT-qPCR fold change = −1.620, p < 0.05) and jejunum (RT-qPCR fold change = −1.549, p < 0.05) 3 months following RYGB, compared to before surgery.

Conclusion

RYGB decreased PUFA ingestion, plasma ALA and EPA levels, and intestinal expression of FADS1 gene. The latter encodes a key enzyme involved in endogenous biosynthesis of PUFAs. These data suggest that supplementation of omega-3 PUFAs may be required for obese patients undergoing RYGB. Clinical Trial Registry number and website: www.clinicaltrials.gov – NCT01251016; Plataforma Brasil – 19339913.0.0000.0068.

0261-5614
1280-1288
Garla, Priscila
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Sala, Priscila
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de Miranda Torrinhas, Raquel Susana Matos
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Mendonça Machado, Natasha
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Fonseca, Danielle Cristina
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Marques da Silva, Mariane
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Ravacci, Graziela Rosa
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Belarmino, Giliane
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Ishida, Robson Kiyoshi
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Guarda, Ismael Francisco Mota Siqueira
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Hourneaux de Moura, Eduardo Guimarães
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Sakai, Paulo
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Santo, Marco Aurélio
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Guerreiro da Silva, Ismael Dale Cotrim
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Pereira, Claudia Cristina Alves
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Heymsfield, Steven
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Corrêa-Giannella, Maria Lúcia Cardillo
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Calder, Philip
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Waitzberg, Dan Linetzky
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Garla, Priscila
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Sala, Priscila
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de Miranda Torrinhas, Raquel Susana Matos
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Mendonça Machado, Natasha
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Fonseca, Danielle Cristina
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Marques da Silva, Mariane
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Ravacci, Graziela Rosa
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Belarmino, Giliane
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Ishida, Robson Kiyoshi
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Guarda, Ismael Francisco Mota Siqueira
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Hourneaux de Moura, Eduardo Guimarães
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Sakai, Paulo
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Santo, Marco Aurélio
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Guerreiro da Silva, Ismael Dale Cotrim
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Pereira, Claudia Cristina Alves
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Heymsfield, Steven
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Corrêa-Giannella, Maria Lúcia Cardillo
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Calder, Philip
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Waitzberg, Dan Linetzky
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Garla, Priscila, Sala, Priscila, de Miranda Torrinhas, Raquel Susana Matos, Mendonça Machado, Natasha, Fonseca, Danielle Cristina, Marques da Silva, Mariane, Ravacci, Graziela Rosa, Belarmino, Giliane, Ishida, Robson Kiyoshi, Guarda, Ismael Francisco Mota Siqueira, Hourneaux de Moura, Eduardo Guimarães, Sakai, Paulo, Santo, Marco Aurélio, Guerreiro da Silva, Ismael Dale Cotrim, Pereira, Claudia Cristina Alves, Heymsfield, Steven, Corrêa-Giannella, Maria Lúcia Cardillo, Calder, Philip and Waitzberg, Dan Linetzky (2019) Reduced intestinal FADS1 gene expression and plasma omega-3 fatty acids following Roux-en-Y gastric bypass. Clinical Nutrition, 38 (3), 1280-1288. (doi:10.1016/j.clnu.2018.05.011).

Record type: Article

Abstract

Background & aims

Roux-en-Y gastric bypass (RYGB) limits food ingestion and may alter the intestinal expression of genes involved in the endogenous synthesis of polyunsaturated fatty acids (PUFAs). These changes may decrease the systemic availability of bioactive PUFAs after RYGB.

Aim

To study the impact of RYGB on the dietary ingestion and plasma concentration of PUFAs and on the intestinal expression of genes involved in their endogenous biosynthesis in severely obese women with type 2 diabetes.

Methods

Before, and 3 and 12 months after RYGB, obese women (n = 20) self-reported a seven-day dietary record, answered a food frequency query and provided plasma samples for alpha-linolenic (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acid assessment by gas chromatography. Intestinal biopsies(duodenum, jejunum and ileum) were collected through double-balloon endoscopybefore and 3 months after RYGB for gene expression analysis by microarray (Human GeneChip 1.0 ST array) and RT-qPCR validation.

Results

Compared to the preoperative period, patients had decreased intakes of PUFAs, fish and soybean oil (p < 0.05) and lower plasma concentrations of ALA and EPA (p < 0.001) 3 and 12 months after RYGB. FADS1 gene expression was lower in duodenum (RT-qPCR fold change = −1.620, p < 0.05) and jejunum (RT-qPCR fold change = −1.549, p < 0.05) 3 months following RYGB, compared to before surgery.

Conclusion

RYGB decreased PUFA ingestion, plasma ALA and EPA levels, and intestinal expression of FADS1 gene. The latter encodes a key enzyme involved in endogenous biosynthesis of PUFAs. These data suggest that supplementation of omega-3 PUFAs may be required for obese patients undergoing RYGB. Clinical Trial Registry number and website: www.clinicaltrials.gov – NCT01251016; Plataforma Brasil – 19339913.0.0000.0068.

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More information

Accepted/In Press date: 21 May 2018
e-pub ahead of print date: 29 May 2018
Published date: 1 June 2019

Identifiers

Local EPrints ID: 421349
URI: http://eprints.soton.ac.uk/id/eprint/421349
ISSN: 0261-5614
PURE UUID: f4c458bd-8762-48c3-943e-5e284292e46f
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 04 Jun 2018 16:30
Last modified: 26 Nov 2021 06:07

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Contributors

Author: Priscila Garla
Author: Priscila Sala
Author: Raquel Susana Matos de Miranda Torrinhas
Author: Natasha Mendonça Machado
Author: Danielle Cristina Fonseca
Author: Mariane Marques da Silva
Author: Graziela Rosa Ravacci
Author: Giliane Belarmino
Author: Robson Kiyoshi Ishida
Author: Ismael Francisco Mota Siqueira Guarda
Author: Eduardo Guimarães Hourneaux de Moura
Author: Paulo Sakai
Author: Marco Aurélio Santo
Author: Ismael Dale Cotrim Guerreiro da Silva
Author: Claudia Cristina Alves Pereira
Author: Steven Heymsfield
Author: Maria Lúcia Cardillo Corrêa-Giannella
Author: Philip Calder ORCID iD
Author: Dan Linetzky Waitzberg

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