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Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age

Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age
Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age
Cortical bone is permeated by a system of pores, occupied by the blood supply and osteocytes. With ageing, bone mass reduction and disruption of the microstructure are associated with reduced vascular supply. Insight into the regulation of the blood supply to the bone could enhance the understanding of bone strength determinants and fracture healing. Using synchrotron radiation-based computed tomography, the distribution of vascular canals and osteocyte lacunae was assessed in murine cortical bone and the influence of age on these parameters was investigated. The tibiofibular junction from 15-week- and 10-month-old female C57BL/6J mice were imaged post-mortem. Vascular canals and three-dimensional spatial relationships between osteocyte lacunae and bone surfaces were computed for both age groups. At 15 weeks, the posterior region of the tibiofibular junction had a higher vascular canal volume density than the anterior, lateral and medial regions. Intracortical vascular networks in anterior and posterior regions were also different, with connectedness in the posterior higher than the anterior at 15 weeks. By 10 months, cortices were thinner, with cortical area fraction and vascular density reduced, but only in the posterior cortex. This provided the first evidence of age-related effects on murine bone porosity due to the location of the intracortical vasculature. Targeting the vasculature to modulate bone porosity could provide an effective way to treat degenerative bone diseases, such as osteoporosis.
281-299
Nunez, Juan, Antonio
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Goring, Alice
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Javaheri, Behzad
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Razi, H.
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Gomez-Nicola, Diego
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Hesse, E.
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Pitsillides, A.A.
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Thurner, Philipp
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Schneider, Philipp
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Clarkin, Claire
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Nunez, Juan, Antonio
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Goring, Alice
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Javaheri, Behzad
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Razi, H.
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Gomez-Nicola, Diego
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Hesse, E.
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Pitsillides, A.A.
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Thurner, Philipp
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Schneider, Philipp
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Clarkin, Claire
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Nunez, Juan, Antonio, Goring, Alice, Javaheri, Behzad, Razi, H., Gomez-Nicola, Diego, Hesse, E., Pitsillides, A.A., Thurner, Philipp, Schneider, Philipp and Clarkin, Claire (2018) Regional diversity in the murine cortical vascular network is revealed by synchrotron X-ray tomography and is amplified with age. European Cells & Materials, 35, 281-299. (doi:10.22203/eCM.v035a20).

Record type: Article

Abstract

Cortical bone is permeated by a system of pores, occupied by the blood supply and osteocytes. With ageing, bone mass reduction and disruption of the microstructure are associated with reduced vascular supply. Insight into the regulation of the blood supply to the bone could enhance the understanding of bone strength determinants and fracture healing. Using synchrotron radiation-based computed tomography, the distribution of vascular canals and osteocyte lacunae was assessed in murine cortical bone and the influence of age on these parameters was investigated. The tibiofibular junction from 15-week- and 10-month-old female C57BL/6J mice were imaged post-mortem. Vascular canals and three-dimensional spatial relationships between osteocyte lacunae and bone surfaces were computed for both age groups. At 15 weeks, the posterior region of the tibiofibular junction had a higher vascular canal volume density than the anterior, lateral and medial regions. Intracortical vascular networks in anterior and posterior regions were also different, with connectedness in the posterior higher than the anterior at 15 weeks. By 10 months, cortices were thinner, with cortical area fraction and vascular density reduced, but only in the posterior cortex. This provided the first evidence of age-related effects on murine bone porosity due to the location of the intracortical vasculature. Targeting the vasculature to modulate bone porosity could provide an effective way to treat degenerative bone diseases, such as osteoporosis.

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Accepted/In Press date: 1 April 2016
e-pub ahead of print date: 23 May 2018
Published date: 23 May 2018

Identifiers

Local EPrints ID: 421523
URI: http://eprints.soton.ac.uk/id/eprint/421523
PURE UUID: 42efa750-6a41-4e16-9ef8-443f8b380f91
ORCID for Diego Gomez-Nicola: ORCID iD orcid.org/0000-0002-5316-2682
ORCID for Philipp Thurner: ORCID iD orcid.org/0000-0001-7588-9041
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

Catalogue record

Date deposited: 14 Jun 2018 16:30
Last modified: 26 Nov 2021 03:01

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Contributors

Author: Juan, Antonio Nunez
Author: Alice Goring
Author: Behzad Javaheri
Author: H. Razi
Author: E. Hesse
Author: A.A. Pitsillides
Author: Philipp Thurner ORCID iD
Author: Claire Clarkin

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