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Cortex Dictamni extracts inhibit over-proliferation and migration of rat airway smooth muscle cells via FAK/p38/Bcl-2 signaling pathway

Cortex Dictamni extracts inhibit over-proliferation and migration of rat airway smooth muscle cells via FAK/p38/Bcl-2 signaling pathway
Cortex Dictamni extracts inhibit over-proliferation and migration of rat airway smooth muscle cells via FAK/p38/Bcl-2 signaling pathway

Airway smooth muscle (ASM) is a prominent effecter in maintaining bronchial muscle contraction and responsible for airway hyper-responsiveness (AHR); the phenotype change and over-proliferation of airway smooth muscle cells (ASMCs) play key roles in the pathogenesis of asthma. The aim of this study was to investigate the anti-proliferation effects of Cortex Dictamni aqueous extract (CDAE) and ethanol extract (CDE) on ASMCs and the possible underline mechanisms. Cell proliferation rates were determined by MTT assay; matrix metalloproteinases-2 (MMP-2) activity was examined by gelatin zymography; cell proliferation and migration were appraised by in-vitro cell-gap closure assessment; protein expressions of p38, Bcl-2 and FAK of ASMCs were evaluated by western blotting and Ca2+ influx of cells was measured by confocal laser microscope. Our data demonstrated that the proliferation, migration and MMP-2 expressions of ASMCs were inhibited by CDAE or CDE; the protein expressions of p38, Bcl-2 and FAK in ASMCs were substantially reduced by CDAE and CDE detected by western blotting or immunocytochemistry; also the increased calcium influx has been observed instantaneously after ASMCs were stimulated by CDAE or CDE. These findings suggested that Cortex Dictamni extracts might have inhibitory effects on ASMCs over-proliferation which could be one of the underline mechanisms for the therapy of asthma.

Airway hyper-responsiveness, Airway smooth muscle cells, Cortex Dictamni extracts
0753-3322
1-8
Wei, Tao
0dc501b0-34af-4335-8776-ffcba47f70ad
Liu, Lei
51ddf4cf-b2db-43ad-8e3b-46f0e110f3b2
Zhou, Xiaoying
84558a96-3129-44de-b295-869d9ee4d19f
Wei, Tao
0dc501b0-34af-4335-8776-ffcba47f70ad
Liu, Lei
51ddf4cf-b2db-43ad-8e3b-46f0e110f3b2
Zhou, Xiaoying
84558a96-3129-44de-b295-869d9ee4d19f

Wei, Tao, Liu, Lei and Zhou, Xiaoying (2018) Cortex Dictamni extracts inhibit over-proliferation and migration of rat airway smooth muscle cells via FAK/p38/Bcl-2 signaling pathway. Biomedicine and Pharmacotherapy, 102, 1-8. (doi:10.1016/j.biopha.2018.03.039).

Record type: Article

Abstract

Airway smooth muscle (ASM) is a prominent effecter in maintaining bronchial muscle contraction and responsible for airway hyper-responsiveness (AHR); the phenotype change and over-proliferation of airway smooth muscle cells (ASMCs) play key roles in the pathogenesis of asthma. The aim of this study was to investigate the anti-proliferation effects of Cortex Dictamni aqueous extract (CDAE) and ethanol extract (CDE) on ASMCs and the possible underline mechanisms. Cell proliferation rates were determined by MTT assay; matrix metalloproteinases-2 (MMP-2) activity was examined by gelatin zymography; cell proliferation and migration were appraised by in-vitro cell-gap closure assessment; protein expressions of p38, Bcl-2 and FAK of ASMCs were evaluated by western blotting and Ca2+ influx of cells was measured by confocal laser microscope. Our data demonstrated that the proliferation, migration and MMP-2 expressions of ASMCs were inhibited by CDAE or CDE; the protein expressions of p38, Bcl-2 and FAK in ASMCs were substantially reduced by CDAE and CDE detected by western blotting or immunocytochemistry; also the increased calcium influx has been observed instantaneously after ASMCs were stimulated by CDAE or CDE. These findings suggested that Cortex Dictamni extracts might have inhibitory effects on ASMCs over-proliferation which could be one of the underline mechanisms for the therapy of asthma.

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More information

Accepted/In Press date: 9 March 2018
e-pub ahead of print date: 13 March 2018
Published date: 1 June 2018
Keywords: Airway hyper-responsiveness, Airway smooth muscle cells, Cortex Dictamni extracts

Identifiers

Local EPrints ID: 421603
URI: http://eprints.soton.ac.uk/id/eprint/421603
ISSN: 0753-3322
PURE UUID: dc488a1f-88dd-42f8-92b6-3daae5f8f83d

Catalogue record

Date deposited: 15 Jun 2018 16:31
Last modified: 15 Mar 2024 19:07

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Contributors

Author: Tao Wei
Author: Lei Liu
Author: Xiaoying Zhou

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