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The requirement for calcification differs between ecologically important coccolithophore species

The requirement for calcification differs between ecologically important coccolithophore species
The requirement for calcification differs between ecologically important coccolithophore species
Coccolithophores are globally distributed unicellular marine algae that are characterized by their covering of calcite coccoliths. Calcification by coccolithophores contributes significantly to global biogeochemical cycles. However, the physiological requirement for calcification remains poorly understood as non‐calcifying strains of some commonly used model species, such as Emiliania huxleyi, grow normally in laboratory culture.
To determine whether the requirement for calcification differs between coccolithophore species, we utilized multiple independent methodologies to disrupt calcification in two important species of coccolithophore: E. huxleyi and Coccolithus braarudii. We investigated their physiological response and used time‐lapse imaging to visualize the processes of calcification and cell division in individual cells.
Disruption of calcification resulted in major growth defects in C. braarudii, but not in E. huxleyi. We found no evidence that calcification supports photosynthesis in C. braarudii, but showed that an inability to maintain an intact coccosphere results in cell cycle arrest.
We found that C. braarudii is very different from E. huxleyi as it exhibits an obligate requirement for calcification. The identification of a growth defect in C. braarudii resulting from disruption of the coccosphere may be important in considering their response to future changes in ocean carbonate chemistry.
0028-646X
147-162
Walker, Charlotte
2ded81ef-5589-43ee-9558-550796b053ce
Taylor, Alison
0551decd-3c1e-4f20-b65d-c8dfab1b4adf
Langer, Gerald
eb30fd90-a855-4699-a383-f7e2d826c084
Durak, Grazyna
b98ebeca-30a0-4775-9553-b8b7b3e8af4e
Heath, Sarah
b443abff-abd0-481e-b792-07559f231795
Probert, Ian
bebae970-4adb-44f6-8101-6790def7dc02
Tyrrell, Toby
6808411d-c9cf-47a3-88b6-c7c294f2d114
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Wheeler, Glen
70608a88-45d6-4d42-968d-00e15c800fd5
Walker, Charlotte
2ded81ef-5589-43ee-9558-550796b053ce
Taylor, Alison
0551decd-3c1e-4f20-b65d-c8dfab1b4adf
Langer, Gerald
eb30fd90-a855-4699-a383-f7e2d826c084
Durak, Grazyna
b98ebeca-30a0-4775-9553-b8b7b3e8af4e
Heath, Sarah
b443abff-abd0-481e-b792-07559f231795
Probert, Ian
bebae970-4adb-44f6-8101-6790def7dc02
Tyrrell, Toby
6808411d-c9cf-47a3-88b6-c7c294f2d114
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Wheeler, Glen
70608a88-45d6-4d42-968d-00e15c800fd5

Walker, Charlotte, Taylor, Alison, Langer, Gerald, Durak, Grazyna, Heath, Sarah, Probert, Ian, Tyrrell, Toby, Brownlee, Colin and Wheeler, Glen (2018) The requirement for calcification differs between ecologically important coccolithophore species. New Phytologist, 147-162. (doi:10.1111/nph.15272).

Record type: Article

Abstract

Coccolithophores are globally distributed unicellular marine algae that are characterized by their covering of calcite coccoliths. Calcification by coccolithophores contributes significantly to global biogeochemical cycles. However, the physiological requirement for calcification remains poorly understood as non‐calcifying strains of some commonly used model species, such as Emiliania huxleyi, grow normally in laboratory culture.
To determine whether the requirement for calcification differs between coccolithophore species, we utilized multiple independent methodologies to disrupt calcification in two important species of coccolithophore: E. huxleyi and Coccolithus braarudii. We investigated their physiological response and used time‐lapse imaging to visualize the processes of calcification and cell division in individual cells.
Disruption of calcification resulted in major growth defects in C. braarudii, but not in E. huxleyi. We found no evidence that calcification supports photosynthesis in C. braarudii, but showed that an inability to maintain an intact coccosphere results in cell cycle arrest.
We found that C. braarudii is very different from E. huxleyi as it exhibits an obligate requirement for calcification. The identification of a growth defect in C. braarudii resulting from disruption of the coccosphere may be important in considering their response to future changes in ocean carbonate chemistry.

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Accepted/In Press date: 7 May 2018
e-pub ahead of print date: 19 June 2018

Identifiers

Local EPrints ID: 421942
URI: https://eprints.soton.ac.uk/id/eprint/421942
ISSN: 0028-646X
PURE UUID: 35af01bb-682e-4152-b5c7-53f9cb07fc07
ORCID for Toby Tyrrell: ORCID iD orcid.org/0000-0002-1002-1716

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Date deposited: 11 Jul 2018 16:30
Last modified: 14 Mar 2019 01:51

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Contributors

Author: Charlotte Walker
Author: Alison Taylor
Author: Gerald Langer
Author: Grazyna Durak
Author: Sarah Heath
Author: Ian Probert
Author: Toby Tyrrell ORCID iD
Author: Colin Brownlee
Author: Glen Wheeler

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