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The characterisation of hepatic mitochondrial function in patients with non-alcoholic fatty liver disease (NAFLD) using the 13C-ketoisocaproate breath test

The characterisation of hepatic mitochondrial function in patients with non-alcoholic fatty liver disease (NAFLD) using the 13C-ketoisocaproate breath test
The characterisation of hepatic mitochondrial function in patients with non-alcoholic fatty liver disease (NAFLD) using the 13C-ketoisocaproate breath test

Hepatic mitochondrial function (HMF) assessed by the 13C-ketoisocaproate breath test (13C-KICA BT) has been previously shown to be significantly associated with the severity of biopsy proven non-alcoholic fatty liver disease (NAFLD). However, it is uncertain whether any perturbation in HMF relates specifically to severity of liver disease or factors associated with metabolic syndrome within non-alcoholic fatty liver disease (NAFLD). Our aim was to investigate whether there was any change in HMF assessed by 13C-KICA BT in patients with NAFLD compared to control subjects, and to assess the factors that are independently associated with HMF.

Methods: 77 patients with NAFLD and 11 healthy control subjects were studied. HMF was assessed using 13C-KICA BT and expressed as cumulative % 13C-dose recovered on breath over 1hr (cPDR over 1hr). Liver fat and fibrosis was assessed by transient elastography. Multi-variable linear regression modelling was undertaken to test the independence of associations with HMF.

Results: HMF (cPDR over 1hr) was lower in NAFLD compared to controls [13.4% (4.8) v. 21.0% (6.3); p<0.0001)]. In NAFLD, HMF was lower in patients with diabetes versus no diabetes [12.7% (3.4) v. 14.3% (6.1); p=0.003)]. Regression modelling showed age (β = -0.08; p=0.01), waist circumference (β = -0.08; p=0.01), hip circumference (β = -0.04; p=0.01), aspartate aminotransferase (AST) (β = -0.05; p=0.01) and diabetes status (β = -1.81; p=0.01) were independently associated with HMF (R2 = 41.5%; p<0.0001).

Conclusions: In patients with NAFLD (compared to healthy subjects), there was a reduction in HMF assessed by the 13C-KICA BT. Furthermore, in patients with NAFLD, HMF is independent and inversely associated with age, waist and hip circumference, AST and diabetes status.

1-7
Afolabi, Paul
757e7f01-664c-493e-bc51-c6a2c933dc22
Scorletti, Eleonora
42bb0659-ac67-4a73-bf36-a881fe6c1563
Smith, Debbie E.
b41c2f97-269c-4a50-ba95-830e823cc7df
Almehmadi, Amal
3721de0d-a04b-4741-af38-0c294f0076af
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher
1370b997-cead-4229-83a7-53301ed2a43c
Afolabi, Paul
757e7f01-664c-493e-bc51-c6a2c933dc22
Scorletti, Eleonora
42bb0659-ac67-4a73-bf36-a881fe6c1563
Smith, Debbie E.
b41c2f97-269c-4a50-ba95-830e823cc7df
Almehmadi, Amal
3721de0d-a04b-4741-af38-0c294f0076af
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Byrne, Christopher
1370b997-cead-4229-83a7-53301ed2a43c

Afolabi, Paul, Scorletti, Eleonora, Smith, Debbie E., Almehmadi, Amal, Calder, Philip and Byrne, Christopher (2018) The characterisation of hepatic mitochondrial function in patients with non-alcoholic fatty liver disease (NAFLD) using the 13C-ketoisocaproate breath test. Journal of Breath Research, 12 (4), 1-7. (doi:10.1088/1752-7163/aacf12).

Record type: Article

Abstract

Hepatic mitochondrial function (HMF) assessed by the 13C-ketoisocaproate breath test (13C-KICA BT) has been previously shown to be significantly associated with the severity of biopsy proven non-alcoholic fatty liver disease (NAFLD). However, it is uncertain whether any perturbation in HMF relates specifically to severity of liver disease or factors associated with metabolic syndrome within non-alcoholic fatty liver disease (NAFLD). Our aim was to investigate whether there was any change in HMF assessed by 13C-KICA BT in patients with NAFLD compared to control subjects, and to assess the factors that are independently associated with HMF.

Methods: 77 patients with NAFLD and 11 healthy control subjects were studied. HMF was assessed using 13C-KICA BT and expressed as cumulative % 13C-dose recovered on breath over 1hr (cPDR over 1hr). Liver fat and fibrosis was assessed by transient elastography. Multi-variable linear regression modelling was undertaken to test the independence of associations with HMF.

Results: HMF (cPDR over 1hr) was lower in NAFLD compared to controls [13.4% (4.8) v. 21.0% (6.3); p<0.0001)]. In NAFLD, HMF was lower in patients with diabetes versus no diabetes [12.7% (3.4) v. 14.3% (6.1); p=0.003)]. Regression modelling showed age (β = -0.08; p=0.01), waist circumference (β = -0.08; p=0.01), hip circumference (β = -0.04; p=0.01), aspartate aminotransferase (AST) (β = -0.05; p=0.01) and diabetes status (β = -1.81; p=0.01) were independently associated with HMF (R2 = 41.5%; p<0.0001).

Conclusions: In patients with NAFLD (compared to healthy subjects), there was a reduction in HMF assessed by the 13C-KICA BT. Furthermore, in patients with NAFLD, HMF is independent and inversely associated with age, waist and hip circumference, AST and diabetes status.

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More information

Accepted/In Press date: 26 June 2018
e-pub ahead of print date: 26 June 2018
Published date: 19 July 2018

Identifiers

Local EPrints ID: 421989
URI: https://eprints.soton.ac.uk/id/eprint/421989
PURE UUID: 53034d8d-cbcd-41a5-99ee-e38a559d4aed
ORCID for Paul Afolabi: ORCID iD orcid.org/0000-0002-0553-1578
ORCID for Christopher Byrne: ORCID iD orcid.org/0000-0001-6322-7753

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Date deposited: 12 Jul 2018 16:30
Last modified: 14 Mar 2019 01:51

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