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Collision cross sections and ion mobility separation of fragment ions from complex N-glycans

Collision cross sections and ion mobility separation of fragment ions from complex N-glycans
Collision cross sections and ion mobility separation of fragment ions from complex N-glycans
Ion mobility mass spectrometry (IM-MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dissociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmentation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.
1044-0305
1250-1261
Harvey, David J.
261201b9-2ec2-4f49-bd32-eadac805da98
Watanabe, Yasunori
8c0ee4af-a293-4de5-9036-3ce2051b380c
Allen, Joel D.
c873d886-2a66-475b-ae04-57a10b37e716
Rudd, Pauline
4bbd1e70-98ae-4c28-84e6-c6a18d98e7ee
Pagel, Kevin
4922aa27-38fc-45d8-9e30-eae9799b874d
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Struwe, Weston B.
16a348b1-3921-4a2d-b5fb-d341fccea65f
Harvey, David J.
261201b9-2ec2-4f49-bd32-eadac805da98
Watanabe, Yasunori
8c0ee4af-a293-4de5-9036-3ce2051b380c
Allen, Joel D.
c873d886-2a66-475b-ae04-57a10b37e716
Rudd, Pauline
4bbd1e70-98ae-4c28-84e6-c6a18d98e7ee
Pagel, Kevin
4922aa27-38fc-45d8-9e30-eae9799b874d
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Struwe, Weston B.
16a348b1-3921-4a2d-b5fb-d341fccea65f

Harvey, David J., Watanabe, Yasunori, Allen, Joel D., Rudd, Pauline, Pagel, Kevin, Crispin, Max and Struwe, Weston B. (2018) Collision cross sections and ion mobility separation of fragment ions from complex N-glycans. Journal of the American Society for Mass Spectrometry, 29 (6), 1250-1261. (doi:10.1007/s13361-018-1930-1).

Record type: Article

Abstract

Ion mobility mass spectrometry (IM-MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dissociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmentation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.

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More information

Accepted/In Press date: 27 February 2018
e-pub ahead of print date: 19 April 2018
Published date: 1 June 2018

Identifiers

Local EPrints ID: 422374
URI: http://eprints.soton.ac.uk/id/eprint/422374
ISSN: 1044-0305
PURE UUID: ec900125-efa2-4dd4-bbe7-df4c4904d725
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 23 Jul 2018 16:30
Last modified: 26 Nov 2021 03:11

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Contributors

Author: David J. Harvey
Author: Yasunori Watanabe
Author: Joel D. Allen
Author: Pauline Rudd
Author: Kevin Pagel
Author: Max Crispin ORCID iD
Author: Weston B. Struwe

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