The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Retinoic acid receptor alpha expression and cutaneous ageing

Retinoic acid receptor alpha expression and cutaneous ageing
Retinoic acid receptor alpha expression and cutaneous ageing

Intrinsic ageing of human skin is a subtle and gradual process that demonstrates few clinical or histological features until old age (>70 years). Initial work indicates that aged skin is "retinoid sensitive" but there is little data on the role of retinoic acid receptors (RARs) or retinoid X receptors (RXRs) in skin ageing. As nuclear retinoid receptors have been implicated in ageing in rodents, we studied the distribution of these receptors in intrinsically aged as compared to young, photoprotected human skin. We found that intrinsic ageing of skin in vivo is accompanied by significant increases of RAR alpha mRNA and protein whereas other isoforms show no alteration with age. In vitro transfection of COS-1 cells with the RAR alpha gene induces expression of matrix metalloproteinase-1 (MMP-1), an enzyme known to play an active role in remodelling of the dermis in intrinsically aged and photoaged skin. Furthermore, addition of all-trans retinoic acid (RA) to cultures of RAR alpha-transfected COS-1 cells diminishes RAR alpha and returns levels of MMP-1 to those approaching baseline. These results demonstrate that intrinsic ageing of human skin is accompanied by significant elevation in the content of RAR alpha and that over-expression of RAR alpha influences expression of MMP-1, an important mediator of skin ageing.

Adult, Aged, Animals, COS Cells, Cellular Senescence, Cercopithecus aethiops, Cyclin-Dependent Kinase Inhibitor p16, Female, Gene Expression Regulation, Developmental, HSP70 Heat-Shock Proteins, Humans, Male, Matrix Metalloproteinase 1, Receptors, Retinoic Acid, Retinoic Acid Receptor alpha, Skin Aging, Tretinoin, Journal Article, Research Support, Non-U.S. Gov't
0047-6374
465-73
Watson, Rachel E B
685f568d-da1c-44ef-a2fa-9ddc1e9145d1
Arjuna Ratnayaka, J
002499b8-1a9f-45b6-9539-5ac145799dfd
Brooke, Rebecca C C
89d893f3-0924-49f2-8662-b225e27da6b3
Yee-Sit-Yu, Sharon
497c0d5c-90b9-4c1c-872c-d9f7ffe25b59
Ancian, Philippe
13c90d84-9c55-4114-810a-7c55157c8bef
Griffiths, Christopher E M
87efcf84-d7c4-4834-a04c-5eeb33dbd12b
Watson, Rachel E B
685f568d-da1c-44ef-a2fa-9ddc1e9145d1
Arjuna Ratnayaka, J
002499b8-1a9f-45b6-9539-5ac145799dfd
Brooke, Rebecca C C
89d893f3-0924-49f2-8662-b225e27da6b3
Yee-Sit-Yu, Sharon
497c0d5c-90b9-4c1c-872c-d9f7ffe25b59
Ancian, Philippe
13c90d84-9c55-4114-810a-7c55157c8bef
Griffiths, Christopher E M
87efcf84-d7c4-4834-a04c-5eeb33dbd12b

Watson, Rachel E B, Arjuna Ratnayaka, J, Brooke, Rebecca C C, Yee-Sit-Yu, Sharon, Ancian, Philippe and Griffiths, Christopher E M (2004) Retinoic acid receptor alpha expression and cutaneous ageing. Mechanisms of Ageing and Development, 125 (7), 465-73. (doi:10.1016/j.mad.2004.03.008).

Record type: Article

Abstract

Intrinsic ageing of human skin is a subtle and gradual process that demonstrates few clinical or histological features until old age (>70 years). Initial work indicates that aged skin is "retinoid sensitive" but there is little data on the role of retinoic acid receptors (RARs) or retinoid X receptors (RXRs) in skin ageing. As nuclear retinoid receptors have been implicated in ageing in rodents, we studied the distribution of these receptors in intrinsically aged as compared to young, photoprotected human skin. We found that intrinsic ageing of skin in vivo is accompanied by significant increases of RAR alpha mRNA and protein whereas other isoforms show no alteration with age. In vitro transfection of COS-1 cells with the RAR alpha gene induces expression of matrix metalloproteinase-1 (MMP-1), an enzyme known to play an active role in remodelling of the dermis in intrinsically aged and photoaged skin. Furthermore, addition of all-trans retinoic acid (RA) to cultures of RAR alpha-transfected COS-1 cells diminishes RAR alpha and returns levels of MMP-1 to those approaching baseline. These results demonstrate that intrinsic ageing of human skin is accompanied by significant elevation in the content of RAR alpha and that over-expression of RAR alpha influences expression of MMP-1, an important mediator of skin ageing.

This record has no associated files available for download.

More information

e-pub ahead of print date: 1 May 2004
Published date: July 2004
Keywords: Adult, Aged, Animals, COS Cells, Cellular Senescence, Cercopithecus aethiops, Cyclin-Dependent Kinase Inhibitor p16, Female, Gene Expression Regulation, Developmental, HSP70 Heat-Shock Proteins, Humans, Male, Matrix Metalloproteinase 1, Receptors, Retinoic Acid, Retinoic Acid Receptor alpha, Skin Aging, Tretinoin, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 422427
URI: http://eprints.soton.ac.uk/id/eprint/422427
ISSN: 0047-6374
PURE UUID: b4f1f593-f8a6-4fd3-9607-8596850d9c1f
ORCID for J Arjuna Ratnayaka: ORCID iD orcid.org/0000-0002-1027-6938

Catalogue record

Date deposited: 24 Jul 2018 16:30
Last modified: 10 Nov 2021 03:34

Export record

Altmetrics

Contributors

Author: Rachel E B Watson
Author: Rebecca C C Brooke
Author: Sharon Yee-Sit-Yu
Author: Philippe Ancian
Author: Christopher E M Griffiths

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×