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Correlation of inflammatory markers of disease with sputum neutrophilia in severe asthma

Correlation of inflammatory markers of disease with sputum neutrophilia in severe asthma
Correlation of inflammatory markers of disease with sputum neutrophilia in severe asthma
Introduction: Sputum inflammometry has been used to describe asthma phenotypes and severe asthma is associated with neutrophilia based on the percentage of cells present. Sputum density is not accounted for in clinical practice.
Methods: Induced sputum was obtained from 110 severe asthmatics (BTS step 4/5) of the Wessex Severe Asthma Cohort. Inflammatory biological markers were measured in sputum supernatant with Multiplex ELISA. Biomarker concentrations were compared to sputum neutrophil density using Spearmans correlation.
Results: The median [IQR] cell number (x 106/g sputum) was significantly lower in P phenotype (n=41 0.64 [0.96]) than N (n=29 2.1 [2.6] (p<0.0005)), E (n=33 1.6[2.9] (p=0.002) and M (n=7 1.8[6.7] (p=0.037)). See table 1. View this table:Table 1 Correlation of sputum neutrophil counts with inflammatory markers
Conclusions: The supernatant measures of MCP1, MIP-1α, TNFα, Eotaxin, GRO α and IL-13 are indicative of the altered biology underlying increased numbers of neutrophils. Relative changes of these markers in response to therapy will help the understanding of the impact of intervention on the biology underlying severe asthma.
0903-1936
Barber, Clair
ff31b460-34c3-466c-90e4-f70b3e954c82
Lau, Laurie
2af8045d-6162-4939-aba7-28dd2f60f6a8
Elliott, Scott
f3234b8e-63df-45b3-a832-26b9a1acaf7f
Ward, Jon
a8107b98-069d-4263-bd70-d29d79f07edc
Gove, Kerry
6493b359-21cd-4068-88f4-a6e684f73316
Brown, Tom
fdd42359-a39d-4f22-b48d-015d9b94ec76
Rupani, Hitasha
cbc7068a-4279-4c8e-b83f-353210711062
Hinks, Tim
42cced71-4ca0-47b9-bb85-619f9fd6ee0d
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Kurukulaaratchy, Ramesh
9c7b8105-2892-49f2-8775-54d4961e3e74
Chauhan, Anoop
4f606144-8948-4f82-8cb1-e57e7a05a8ed
Staples, Karl
e0e9d80f-0aed-435f-bd75-0c8818491fee
Howarth, Peter
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Barber, Clair
ff31b460-34c3-466c-90e4-f70b3e954c82
Lau, Laurie
2af8045d-6162-4939-aba7-28dd2f60f6a8
Elliott, Scott
f3234b8e-63df-45b3-a832-26b9a1acaf7f
Ward, Jon
a8107b98-069d-4263-bd70-d29d79f07edc
Gove, Kerry
6493b359-21cd-4068-88f4-a6e684f73316
Brown, Tom
fdd42359-a39d-4f22-b48d-015d9b94ec76
Rupani, Hitasha
cbc7068a-4279-4c8e-b83f-353210711062
Hinks, Tim
42cced71-4ca0-47b9-bb85-619f9fd6ee0d
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Kurukulaaratchy, Ramesh
9c7b8105-2892-49f2-8775-54d4961e3e74
Chauhan, Anoop
4f606144-8948-4f82-8cb1-e57e7a05a8ed
Staples, Karl
e0e9d80f-0aed-435f-bd75-0c8818491fee
Howarth, Peter
ff19c8c4-86b0-4a88-8f76-b3d87f142a21

Barber, Clair, Lau, Laurie, Elliott, Scott, Ward, Jon, Gove, Kerry, Brown, Tom, Rupani, Hitasha, Hinks, Tim, Djukanovic, Ratko, Kurukulaaratchy, Ramesh, Chauhan, Anoop, Staples, Karl and Howarth, Peter (2015) Correlation of inflammatory markers of disease with sputum neutrophilia in severe asthma. European Respiratory Journal, 46 (Suppl 59).

Record type: Meeting abstract

Abstract

Introduction: Sputum inflammometry has been used to describe asthma phenotypes and severe asthma is associated with neutrophilia based on the percentage of cells present. Sputum density is not accounted for in clinical practice.
Methods: Induced sputum was obtained from 110 severe asthmatics (BTS step 4/5) of the Wessex Severe Asthma Cohort. Inflammatory biological markers were measured in sputum supernatant with Multiplex ELISA. Biomarker concentrations were compared to sputum neutrophil density using Spearmans correlation.
Results: The median [IQR] cell number (x 106/g sputum) was significantly lower in P phenotype (n=41 0.64 [0.96]) than N (n=29 2.1 [2.6] (p<0.0005)), E (n=33 1.6[2.9] (p=0.002) and M (n=7 1.8[6.7] (p=0.037)). See table 1. View this table:Table 1 Correlation of sputum neutrophil counts with inflammatory markers
Conclusions: The supernatant measures of MCP1, MIP-1α, TNFα, Eotaxin, GRO α and IL-13 are indicative of the altered biology underlying increased numbers of neutrophils. Relative changes of these markers in response to therapy will help the understanding of the impact of intervention on the biology underlying severe asthma.

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More information

Published date: September 2015
Venue - Dates: European Respiratory Society Annual Congress, Amsterdam, Netherlands, 2015-09-26 - 2015-09-30

Identifiers

Local EPrints ID: 422521
URI: https://eprints.soton.ac.uk/id/eprint/422521
ISSN: 0903-1936
PURE UUID: c19d0553-8e9b-4678-b466-51da1260bbb4
ORCID for Clair Barber: ORCID iD orcid.org/0000-0001-5335-5129
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612
ORCID for Karl Staples: ORCID iD orcid.org/0000-0003-3844-6457

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Date deposited: 25 Jul 2018 16:30
Last modified: 14 Mar 2019 01:55

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