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CD48 on blood leukocytes and in serum of asthma patients varies with severity

CD48 on blood leukocytes and in serum of asthma patients varies with severity
CD48 on blood leukocytes and in serum of asthma patients varies with severity
Background: CD48 is a membrane receptor (mCD48) on eosinophils and mast cells and exists in a soluble form (sCD48). CD48 has a pivotal role in murine asthma and in the proinflammatory interactions of mast cells with eosinophils via its ligand CD244. Thus, CD48 might be important in human asthma. 
Methods: Therefore, two separate cohorts (IL and UK) comprising mild, moderate, and severe asthma and healthy volunteers were evaluated for blood leukocyte mCD48 expression and sCD48 in serum. Asthmatic bronchial biopsies were immunostained for CD48. sCD48 effect on CD244‐dependent eosinophil activation was evaluated. 
Results: Eosinophil mCD48 expression was significantly elevated in moderate while downregulated in severe asthma. mCD48 expression on B, T, and NK cells and monocytes in severe asthma was significantly increased. sCD48 levels were significantly higher in mild while reduced in severe asthma. sCD48 optimal cutoff values for differentiating asthma from health were identified as >1482 pg/ml (IL) and >1619 pg/ml (UK). In asthmatic bronchial biopsies, mCD48 was expressed predominantly by eosinophils. sCD48 inhibited anti‐CD244‐induced eosinophil activation. 
Conclusions: mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244‐mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma.
0105-4538
888-895
Gangwar, Roopesh S.
2934e197-5d5f-4328-9401-75cef02457e6
Minai-Fleminger, Y.
f41f1f5a-5b8f-4ab2-9240-f0ee82b3b71f
Seaf, M.
4a7f4718-7184-4347-b374-4a472b362771
Gutcold, A.
92f73da2-60f3-4d1e-bb2b-9d4159676f5c
Shikotra, A.
81f10100-8d6c-48a9-8c15-684a62fb9875
Barber, C.
ff31b460-34c3-466c-90e4-f70b3e954c82
Chauhan, A.
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Holgate, S.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Bradding, P.
39990c99-3c21-44b6-b1f7-084eddf29722
Howarth, P.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Elishar, R.
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Berkman, N.
c74901d4-b4c4-45dc-a5c5-036824afa4c9
Levi_Schaffer, F.
9cb19b8e-ef58-475e-a070-c5237c08eaf7
Gangwar, Roopesh S.
2934e197-5d5f-4328-9401-75cef02457e6
Minai-Fleminger, Y.
f41f1f5a-5b8f-4ab2-9240-f0ee82b3b71f
Seaf, M.
4a7f4718-7184-4347-b374-4a472b362771
Gutcold, A.
92f73da2-60f3-4d1e-bb2b-9d4159676f5c
Shikotra, A.
81f10100-8d6c-48a9-8c15-684a62fb9875
Barber, C.
ff31b460-34c3-466c-90e4-f70b3e954c82
Chauhan, A.
4f606144-8948-4f82-8cb1-e57e7a05a8ed
Holgate, S.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Bradding, P.
39990c99-3c21-44b6-b1f7-084eddf29722
Howarth, P.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Elishar, R.
66ca7a8f-29df-4089-a5f5-f855689485a8
Berkman, N.
c74901d4-b4c4-45dc-a5c5-036824afa4c9
Levi_Schaffer, F.
9cb19b8e-ef58-475e-a070-c5237c08eaf7

Gangwar, Roopesh S., Minai-Fleminger, Y., Seaf, M., Gutcold, A., Shikotra, A., Barber, C., Chauhan, A., Holgate, S., Bradding, P., Howarth, P., Elishar, R., Berkman, N. and Levi_Schaffer, F. (2017) CD48 on blood leukocytes and in serum of asthma patients varies with severity. Allergy, 72 (6), 888-895. (doi:10.1111/all.13082).

Record type: Article

Abstract

Background: CD48 is a membrane receptor (mCD48) on eosinophils and mast cells and exists in a soluble form (sCD48). CD48 has a pivotal role in murine asthma and in the proinflammatory interactions of mast cells with eosinophils via its ligand CD244. Thus, CD48 might be important in human asthma. 
Methods: Therefore, two separate cohorts (IL and UK) comprising mild, moderate, and severe asthma and healthy volunteers were evaluated for blood leukocyte mCD48 expression and sCD48 in serum. Asthmatic bronchial biopsies were immunostained for CD48. sCD48 effect on CD244‐dependent eosinophil activation was evaluated. 
Results: Eosinophil mCD48 expression was significantly elevated in moderate while downregulated in severe asthma. mCD48 expression on B, T, and NK cells and monocytes in severe asthma was significantly increased. sCD48 levels were significantly higher in mild while reduced in severe asthma. sCD48 optimal cutoff values for differentiating asthma from health were identified as >1482 pg/ml (IL) and >1619 pg/ml (UK). In asthmatic bronchial biopsies, mCD48 was expressed predominantly by eosinophils. sCD48 inhibited anti‐CD244‐induced eosinophil activation. 
Conclusions: mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244‐mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma.

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More information

Accepted/In Press date: 7 November 2016
e-pub ahead of print date: 10 November 2016
Published date: June 2017

Identifiers

Local EPrints ID: 422568
URI: https://eprints.soton.ac.uk/id/eprint/422568
ISSN: 0105-4538
PURE UUID: 2e11bf35-6bd8-40a8-8b23-50742cd16da0
ORCID for C. Barber: ORCID iD orcid.org/0000-0001-5335-5129

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Date deposited: 25 Jul 2018 16:30
Last modified: 10 Dec 2019 01:29

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Contributors

Author: Roopesh S. Gangwar
Author: Y. Minai-Fleminger
Author: M. Seaf
Author: A. Gutcold
Author: A. Shikotra
Author: C. Barber ORCID iD
Author: A. Chauhan
Author: S. Holgate
Author: P. Bradding
Author: P. Howarth
Author: R. Elishar
Author: N. Berkman
Author: F. Levi_Schaffer

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