Spindle tubulin and MTOC asymmetries may explain meiotic drive in oocytes
Spindle tubulin and MTOC asymmetries may explain meiotic drive in oocytes
In the first meiotic division (MI) of oocytes, the cortically positioned spindle causes bivalent segregation in which only the centre-facing homolog pairs are retained. ‘Selfish’ chromosomes are known to exist, which bias their spindle orientation and hence retention in the egg, a process known as ‘meiotic drive’. Here we report on this phenomenon in oocytes from F1 hybrid mice, where parental strain differences in centromere size allows distinction of the two homolog pairs of a bivalent. Bivalents with centromere and kinetochore asymmetry show meiotic drive by rotating during prometaphase, in a process dependent on aurora kinase activity. Cortically positioned homolog pairs appear to be under greater stretch than their centre-facing partners. Additionally the cortex spindle-half contain a greater density of tubulin and microtubule organising centres. A model is presented in which meiotic drive is explained by the impact of microtubule force asymmetry on chromosomes with different sized centromeres and kinetochores.
1-11
Wu, Tianyu
90a93711-118f-485d-9f50-96c1cc94f48e
Lane, Simon I. R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Morgan, Stephanie L.
3f975d1c-0af5-4e8e-871b-359733ff4026
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
27 July 2018
Wu, Tianyu
90a93711-118f-485d-9f50-96c1cc94f48e
Lane, Simon I. R.
8e80111f-5012-4950-a228-dfb8fb9df52d
Morgan, Stephanie L.
3f975d1c-0af5-4e8e-871b-359733ff4026
Jones, Keith T.
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Wu, Tianyu, Lane, Simon I. R., Morgan, Stephanie L. and Jones, Keith T.
(2018)
Spindle tubulin and MTOC asymmetries may explain meiotic drive in oocytes.
Nature Communications, 9 (1), , [2952].
(doi:10.1038/s41467-018-05338-7).
Abstract
In the first meiotic division (MI) of oocytes, the cortically positioned spindle causes bivalent segregation in which only the centre-facing homolog pairs are retained. ‘Selfish’ chromosomes are known to exist, which bias their spindle orientation and hence retention in the egg, a process known as ‘meiotic drive’. Here we report on this phenomenon in oocytes from F1 hybrid mice, where parental strain differences in centromere size allows distinction of the two homolog pairs of a bivalent. Bivalents with centromere and kinetochore asymmetry show meiotic drive by rotating during prometaphase, in a process dependent on aurora kinase activity. Cortically positioned homolog pairs appear to be under greater stretch than their centre-facing partners. Additionally the cortex spindle-half contain a greater density of tubulin and microtubule organising centres. A model is presented in which meiotic drive is explained by the impact of microtubule force asymmetry on chromosomes with different sized centromeres and kinetochores.
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2018_NatureComm
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Accepted/In Press date: 30 June 2018
e-pub ahead of print date: 27 July 2018
Published date: 27 July 2018
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Local EPrints ID: 422684
URI: http://eprints.soton.ac.uk/id/eprint/422684
PURE UUID: d8443d4a-1a79-4a6d-815a-d87dffb51646
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Date deposited: 30 Jul 2018 16:30
Last modified: 16 Mar 2024 04:15
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Author:
Tianyu Wu
Author:
Simon I. R. Lane
Author:
Stephanie L. Morgan
Author:
Keith T. Jones
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