Prospective use of high-refractive index materials for single molecule detection in flow cytometry
Prospective use of high-refractive index materials for single molecule detection in flow cytometry
Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry.
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Horak, Peter
520489b5-ccc7-4d29-bb30-c1e36436ea03
1 August 2018
Horak, Peter
520489b5-ccc7-4d29-bb30-c1e36436ea03
Horak, Peter
(2018)
Prospective use of high-refractive index materials for single molecule detection in flow cytometry.
Sensors, 18 (8), , [2461].
(doi:10.3390/s18082461).
Abstract
Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry.
Text
single molecule detection flow cytometry sensors
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Submitted date: 23 April 2018
Accepted/In Press date: 18 July 2018
e-pub ahead of print date: 1 August 2018
Published date: 1 August 2018
Identifiers
Local EPrints ID: 422721
URI: http://eprints.soton.ac.uk/id/eprint/422721
ISSN: 1424-8220
PURE UUID: f4b56dbf-f8da-4e35-80e0-c7e1ae075634
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Date deposited: 01 Aug 2018 16:30
Last modified: 16 Mar 2024 03:27
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Author:
Peter Horak
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