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Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs

Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs
Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs
We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the applicability of this technique for studying the effect such platforms render on neonatal rat ventricular myocytes (NRVMs). Firstly, we have characterised topographically and mechanically the scaffolds in liquid at the nano-scale level. We thus establish that such platforms have physical properties that closely mimics the in vivo extracellular environment of cells. We have then studied the cell morphology and physiology by comparing cultures on flat uniformly-covered and collagen-patterned scaffolds. We show that micro-patterns promote the elongation of cells along the principal axis of the ridges coated with collagen. In several cases, cells also tend to create bridges across the grooves. We have finally studied cell contraction, monitoring Ca2+ cycling at a certain stimulation. Cells seeded on patterned scaffolds present significant responses in comparison to the isotropic ones.
2045-2322
1-12
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Humphrey, E.J.
d728a181-61c5-4b44-9783-e94c6c95a5c6
Dinelli, F
0316eeb9-41bd-4feb-abc1-aac06704f46e
Terracciano, C.
b8db4556-6cd3-4dac-b667-2924e3bf8ce5
Prodromakis, Themis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Humphrey, E.J.
d728a181-61c5-4b44-9783-e94c6c95a5c6
Dinelli, F
0316eeb9-41bd-4feb-abc1-aac06704f46e
Terracciano, C.
b8db4556-6cd3-4dac-b667-2924e3bf8ce5
Prodromakis, Themis
d58c9c10-9d25-4d22-b155-06c8437acfbf

Sanzari, Ilaria, Humphrey, E.J., Dinelli, F, Terracciano, C. and Prodromakis, Themis (2018) Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs. Scientific Reports, 8, 1-12. (doi:10.1038/s41598-018-30360-6).

Record type: Article

Abstract

We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the applicability of this technique for studying the effect such platforms render on neonatal rat ventricular myocytes (NRVMs). Firstly, we have characterised topographically and mechanically the scaffolds in liquid at the nano-scale level. We thus establish that such platforms have physical properties that closely mimics the in vivo extracellular environment of cells. We have then studied the cell morphology and physiology by comparing cultures on flat uniformly-covered and collagen-patterned scaffolds. We show that micro-patterns promote the elongation of cells along the principal axis of the ridges coated with collagen. In several cases, cells also tend to create bridges across the grooves. We have finally studied cell contraction, monitoring Ca2+ cycling at a certain stimulation. Cells seeded on patterned scaffolds present significant responses in comparison to the isotropic ones.

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Accepted/In Press date: 30 July 2018
e-pub ahead of print date: 10 August 2018
Published date: 2018

Identifiers

Local EPrints ID: 422722
URI: https://eprints.soton.ac.uk/id/eprint/422722
ISSN: 2045-2322
PURE UUID: f50f261e-501e-423e-9173-71dbcf09d7e5
ORCID for Themis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 01 Aug 2018 16:30
Last modified: 23 May 2019 00:31

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