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Innate-like functions of natural killer T cell subsets result from highly divergent gene programs

Innate-like functions of natural killer T cell subsets result from highly divergent gene programs
Innate-like functions of natural killer T cell subsets result from highly divergent gene programs

Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of the differential expression of a few transcription factors and cell-surface molecules. Here we have analyzed purified populations of thymic NKT cell subsets at both the transcriptomic level and epigenomic level and by single-cell RNA sequencing. Our data indicated that despite their similar antigen specificity, the functional NKT cell subsets were highly divergent populations with many gene-expression and epigenetic differences. Therefore, the thymus 'imprints' distinct gene programs on subsets of innate-like NKT cells that probably impart differences in proliferative capacity, homing, and effector functions.

Animals, Antigens, CD1d, Cell Movement, Cell Proliferation, Cells, Cultured, Epigenesis, Genetic, Gene Expression Regulation, Immunity, Innate, Jumonji Domain-Containing Histone Demethylases, Lymphocyte Subsets, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Natural Killer T-Cells, Sequence Analysis, RNA, Single-Cell Analysis, Thymus Gland, Transcriptome, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
1529-2908
728-739
Engel, Isaac
ca31a235-98e5-4311-8be1-b9a4e123edec
Seumois, Grégory
0be7d3d6-5526-458c-aa5c-cce52410a2ed
Chavez, Lukas
84f58e4d-a3e4-4b69-87d4-5783f15580cb
Samaniego-Castruita, Daniela
e0925b0f-9b07-46e3-a433-08a941e11c6f
White, Brandie
f855d63c-b21e-4def-93f5-9f2f18ac13f9
Chawla, Ashu
a7528db3-560b-4688-ad38-cbf077bfffb7
Mock, Dennis
3fe0ef07-4185-48e1-bab1-b1280bab4c41
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Kronenberg, Mitchell
80aa71cb-4e8e-46b9-95c9-2cabedeb7bf0
Engel, Isaac
ca31a235-98e5-4311-8be1-b9a4e123edec
Seumois, Grégory
0be7d3d6-5526-458c-aa5c-cce52410a2ed
Chavez, Lukas
84f58e4d-a3e4-4b69-87d4-5783f15580cb
Samaniego-Castruita, Daniela
e0925b0f-9b07-46e3-a433-08a941e11c6f
White, Brandie
f855d63c-b21e-4def-93f5-9f2f18ac13f9
Chawla, Ashu
a7528db3-560b-4688-ad38-cbf077bfffb7
Mock, Dennis
3fe0ef07-4185-48e1-bab1-b1280bab4c41
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Kronenberg, Mitchell
80aa71cb-4e8e-46b9-95c9-2cabedeb7bf0

Engel, Isaac, Seumois, Grégory, Chavez, Lukas, Samaniego-Castruita, Daniela, White, Brandie, Chawla, Ashu, Mock, Dennis, Vijayanand, Pandurangan and Kronenberg, Mitchell (2016) Innate-like functions of natural killer T cell subsets result from highly divergent gene programs. Nature Immunology, 17 (6), 728-739. (doi:10.1038/ni.3437).

Record type: Article

Abstract

Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of the differential expression of a few transcription factors and cell-surface molecules. Here we have analyzed purified populations of thymic NKT cell subsets at both the transcriptomic level and epigenomic level and by single-cell RNA sequencing. Our data indicated that despite their similar antigen specificity, the functional NKT cell subsets were highly divergent populations with many gene-expression and epigenetic differences. Therefore, the thymus 'imprints' distinct gene programs on subsets of innate-like NKT cells that probably impart differences in proliferative capacity, homing, and effector functions.

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More information

Accepted/In Press date: 15 March 2016
e-pub ahead of print date: 18 April 2016
Keywords: Animals, Antigens, CD1d, Cell Movement, Cell Proliferation, Cells, Cultured, Epigenesis, Genetic, Gene Expression Regulation, Immunity, Innate, Jumonji Domain-Containing Histone Demethylases, Lymphocyte Subsets, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Natural Killer T-Cells, Sequence Analysis, RNA, Single-Cell Analysis, Thymus Gland, Transcriptome, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 422822
URI: https://eprints.soton.ac.uk/id/eprint/422822
ISSN: 1529-2908
PURE UUID: ccb8d50e-1f73-4ce1-889d-5ca1115d1532

Catalogue record

Date deposited: 06 Aug 2018 16:30
Last modified: 06 Aug 2018 16:30

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